Poster Topical Area: Experimental Animal Nutrition

Location: Hall D

Poster Board Number: 619

P11-007 - Cats vary circulating metabolomics in response to dietary fatty acids

Monday, Jun 11
8:00 AM – 3:00 PM

Objectives: This study evaluated the circulating metabolomics response of cats to changing dietary fatty acids.
Methods: Thirty three pets were first fed a food containing 0.09% arachidonic acid (AA) and EPA less than 0.01% for 28 days. Cats were than assigned into three groups: Group 1 (n=11) was fed a food containing 0.10% AA and 0.23% EPA, group 2 (n=11) was fed a food containing 0.08% AA and less than 0.01% EPA, group 3 (n=11) was fed a food containing 0.16% AA and less than 0.01% EPA. Test foods were fed for 56 days. All study protocols were reviewed and approved by the Institutional Animal Care and Use Committee, Hill's Pet Nutrition, Inc., Topeka, KS. Metabolomics were measured by Metabolon (Morrisville, NC), fatty acids were measured by liquid chromatography. Initial metabolomics and fatty acid profiles were compared to final and compared across treatments.
Results: Arachidonic acid was initially similar in all groups (21 + 1.4 mg/dl) and then different (p<0.05) at the end of the 56 day feeding period (17, 22, and 31 + 1.4, for groups 1, 2, and 3 respectively). There was a direct effect of food on circulating EPA concentration with all pets starting at similar concentrations (0.51 + 0.03 mg/dl) with only the supplemented group increasing (p<0.05) concentration (5.39, 0.56, 0.59 + 0.38 mg/dl for groups 1, 2 and 3 respectively) at the end of the 56 day study. Concentrations of AA and EPA were similar within groups at 28 and 56 days. Acyl carnitines of EPA were increased in group 1. With the addition of EPA, octanoylcarnitine (C8), myristoylcarnitine (C14), nervonoylcarnitine (C24:1), cerotoylcarnitine (C26), and ximenoylcarnitine (C26:1) were increased (P<0.05). Similarly, arachidonoyl carnitine (C20:4) and adrenoyl carnitine (C22:4) were higher (p<0.05) in the AA group relative to the others. There was no change in circulating carnitine or deoxycarnitine. The dietary increase of either AA or EPA resulted in reduced circulating concentrations of methionine, methionine sulfone and methionine sulfoxide. Cats fed increased EPA had higher levels of betaine and dimethylglycine.
Conclusions: These data show that cats have both direct and indirect responses to changing dietary fatty acids.




Funding Source: Hill's Pet Nutrition, Inc.

CoAuthors: Matthew Jackson – Hills Pet Nutrition Inc

Dennis E. Jewell

Senior Fellow
Hill's Pet Nutrition, Inc
Topeka, Kansas