Poster Topical Area: Energy and Macronutrient Metabolism

Location: Hall D

Poster Board Number: 503

P10-104 - Dietary PUFA and postprandial total cholesterol and triglyceride concentrations

Monday, Jun 11
8:00 AM – 3:00 PM

Objectives: The objective of this work was to determine the impact of the consumption of different dietary fatty acids on postprandial (PP) lipemia. We hypothesized that intake of unsaturated fatty acids results in lower TG and total cholesterol (TC) concentrations in the PP state compared to SFA.

With preliminary data from an ongoing study, we examinedPP lipid responses over 4 hours in volunteer participants. The trial was a cross over-designed, acute study in which participants consumed each of five treatments (SFA, MUFA, PUFA-ALA, PUFA-LA, and PUFA- long chain omega-3 (PUFA-LCn3)) in random order with a one week washout between treatments. Participants were admitted to the research center the evening before testing where they received a standardized dinner meal and were NPO until testing. A fat tolerance test, provided as a beverage containing 30 g of total fat and enriched in the fatty acids of interest, was provided after the time 0 blood draw. Concentrations of TC, LDL-C, HDL-C, and TG were determined at 0, 30, 60, 120, 180, and 240 minutes. The area under the curve (AUC; mg/dL/hr) corrected for baseline for each lipoprotein was calculated by the trapezoid rule.

Overweight to obese men (n=2) and women (n=6) aged 26.1 2.3 (mean SEM) with a BMI of 30.9 ± 0.8 kg/m2 are included in this analysis from an ongoing study with a total n=22. No differences were observed in the AUC for HDL-C, or LDL-C. The TC AUC was significantly reduced with PUFA-LCn3 compared to SFA (p=0.02). The TG AUC was significant reduced with PUFA-ALA, PUFA-LA, and PUFA-LCn-3 intake compared to SFA (p=0.001, p=0.05, and p=0.01, respectively).

Our preliminary data provide evidence that PP TG and TC AUC are reduced after consuming test meals which contain PUFA compared to SFA. Further investigations are needed to determine day long interaction of dietary fatty acids with postprandial lipemia.

Funding Source:

This research was supported by the USDA, ARS project 3062-51000-053-00D.

CoAuthors: Vanessa Thyne, BS – USDA; LuAnn Johnson, MS – USDA; Matthew Picklo, PhD – USDA

Susan K. Raatz

Research Nutritionist
Grand Forks, North Dakota