Poster Topical Area: Aging and Chronic Disease
Poster Board Number: 44
Objectives: Gastrointestinal diseases and symptoms are prevalent in patients with Chronic Obstructive Pulmonary Disease (COPD), however the exact disturbances in gut function as well as its functional consequences remain unclear. This study investigates whether an impaired protein digestion capacity is present in COPD patients and, if so, to what extent it is associated with a reduced anabolic response to feeding and quality of life.
Methods: In 23 patients with moderate to severe COPD (GOLD stage II-IV) and 17 healthy control subjects, the stable tracers of Phenylalanine (PHE)-[ring-2H5] and Tyrosine (TYR)-[13C9,15N] were administered intravenously via primed constant and continuous infusion for 6 hours. Two hours after start of the infusion, a complete high protein meal containing PHE-[1-13C] and Spirulina-[U-15N] was ingested. Blood samples were taken and enrichments analysed by LC-MS/MS. The Spirulina-[U-15N] degradation ratio, as marker of protein digestion capacity, was calculated as the ratio of whole body rate of appearance of PHE-[15N] from Spirulina-[U-15N] to that of PHE-[1-13C]. The anabolic response to feeding was calculated both corrected and uncorrected for protein digestion capacity, and disease characteristics and outcome measures were assessed. Statistics was done by unpaired t-tests.
Texas A&M University
College Station, Texas