Poster Topical Area: Experimental Animal Nutrition

Location: Hall D

Poster Board Number: 616

P11-003 - Anti-aging Food that Imparts Health Benefits to senior Dogs by Modulating the Gut Microbiome and Metabolites

Monday, Jun 11
8:00 AM – 3:00 PM

Objectives: This study evaluates the effects of a test food containing components of citrus, carrot, spinach and tomato on the gut microbiome and age-related metabolites in senior dogs.

Methods: Thirty-six dogs between 8 and 13 years of age were maintained on a control food (control 1) containing none of the fiber sources present in the test or the control 2 foods. Thirty days later, the dogs were divided into two groups of 18 dogs. During the next 30 days, each group received either the test or the control 2 food, which contained whole grains but none of the above fiber sources in the test food. Both groups consumed the control 1 food for the next 30 days. A crossover was performed to feed the food which had not yet been fed to the dogs (test or control 2) for another 30 days. Samples from feces and blood were collected at the end of each 30 days period to analyze changes in gut microbial composition and fecal and circulating metabolites.

Results: The test food increased proportions of the genera Adlercreutzia, Oscillospira, Phascolarcobacteria, Faecalibacterium and Ruminococcus and reduced Megamonas, Salmonella and Peptostreptococcus. At the family level, the consumption of the test food increased proportions of Christensenellaceae and Ruminococcaceae while reducing Enterobacteriaceae. The phylum Fusobacterium declined while Cyanobacteria and Acidobacteria increased after the consumption of the test food. The test food led to a significant reduction in fecal levels of the advanced glycation end product (AGE), pyrraline and the mucin amino acids; threonine, serine, aspargine and proline. On contrary, the test food increased the relative level of glycerol and fatty acids in feces. The dogs had lower levels of circulating pyrraline and phenolic uremic toxins, including the microbial brain toxin 4-ethylphenyl sulfate (4-EPS). The test food also led to a significant improvement in the renal health marker, symmetric dimethylarginine (SDMA).

Conclusions: Fermentable fibers from fruit and vegetables enhance health in senior dogs by increasing the proportions of beneficial gut bacteria and reducing levels of toxic metabolites involved in aging, kidney, brain and gut health.

Funding Source: The work presented in this study was funded by Hill's Pet Nutrition, Topeka, KS. (

CoAuthors: Matthew Jackson, PhD – Hill's Pet Nutrition; Dennis Jewell, PhD – Hill's Pet Nutrition

Eden Ephraim Gebreselassie

Senior Scientist, Nutrition Innovation
Hill's Pet Nutrition
Topeka, Kansas