Poster Topical Area: Carotenoids and Retinoids

Location: Auditorium

Poster Board Number: 12

P03-004 - β-carotene regulated colon cancer stem cells by suppressing cancer stemness.

Sunday, Jun 10
8:00 AM – 6:00 PM

Objective: Colorectal cancer (CRC), the third most common cancer in worldwide, has high rate of recurrence and metastasis. These characteristics can be explained by cancer stem cells (CSCs). CSCs are the small population of cancer cells that possess characteristics of stem cells. β-carotene (BC) has reported to be effective on suppressing CSCs in neuroblastoma. The object of this study was to analyze anti-CSCs effect of BC in colorectal cancer.


Methods:
To measure the self-renewal capacity, clonogenic assays and sphere formation assays were used. CSC markers and Wnt/β-catenin signaling pathway were analyzed in CD133+CD44+ HCT 116 and HT29 colon cancer cells and primary cells from human CRC tissues. The xenograft model was used to investigate the tumorigenicity of CSC in mice after 10 weeks of BC supplementation.

Result: CSCs were isolated after double staining of CD133 and CD44. BC reduced the number and size of colonies and spheres, which indicated the anti-self renewal capacity of BC. BC also down-regulated CSC markers, including CD44, CD133, ALDH1A1,NOTCH1,and Sox2, and Wnt/β-catenin signaling pathway, including β-catenin in CD133+CD44+ HCT116 and HT-29 cells. In primary cells isolated from human CRC, anti-CSCs effect of BC was confirmed. In addition, the number and size of tumors were decreased and the time of onset of tumor was delayed by BC supplementation in xenograft mice injected with CD133+CD44+ HCT116 cells. BC also inhibited CSCs markers and Wnt/β-catenin signaling pathway in tumors in vivo.


Conclusions:
BC exerted inhibitory effect on colon CSC by regulating cancer stemness which indicated its therapeutic potential in CRC.




Funding Source: This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2016R1D1A1B03932018) and Brain Korea 21 Plus (Project Number: 22A20130012143).

CoAuthors: Kyung Eun Lee – Department of Nutritional Science and Food Management, Ewha Womans University; Yoo Sun Kim – Department of Nutritional Science and Food Management, Ewha Womans University; Seung Chul Heo – Department of Surgery, Seoul National University Boramae Medical Center; Yuri Kim – Department of Nutritional Science and Food Management, Ewha Womans University

Na Youn Lee

graduate student
Department of Nutritional Science and Food Management, Ewha Womans University
Seoul, Seoul-t'ukpyolsi, Republic of Korea