Poster Topical Area: Nutritional Immunology
Location: Hall D
Poster Board Number: 839
Hemorrhagic shock and resuscitation has been considered as a condition with inflammation and immune dysfunction. We previously showed that amino acid supplemented-resuscitation fluids have beneficial effects on hematology and organ inflammatory response in rats with trauma-hemorrhagic shock and resuscitation (THR). The aim of this study was to investigate the immunomodulatory activity of different amino acid-supplemented resuscitation fluids on immunocytes in THR. Male SD rats were performed with carotid arterial and jugular vein cannulations only (SHA group) or with 5 cm midline laparotomy and blood loss to mean arterial pressure at 35 mmHg. After 60 minutes, rats were resuscitated with shed blood and equal volume of lactate Ringer's solution without (THR group) or with equal nitrogenous L-alanyl-L-glutamine (GLN group), L-arginine plus L-alanine (ARG group), or L-amino acid mixture plus L-alanine (AMX group) within 10 minutes and followed by continuous infusion for 42 h (~1.3 ml/h). The results showed that the THR-increased circulating granulocytes and splenocyte proliferation were significantly decreased in the ARG and GLN groups, respectively (one-way ANOVA, p<0.05). The THR-decreased splenocytic phagocytosis was significantly increased in the GLN and AMX groups. The THR-induced increase in TNF-alpha secretion in the splenocytes was significantly reversed in the ARG, GLN, and AMX groups and that of IL-6 secretion was reversed in the ARG and GLN groups. Moreover, the GLN and AMX groups had significantly decreased IL-4, IL-6, and IFN-gamma secretion from the peripheral blood leukocytes (PBLs) and decreased IFN-gamma and IL-10 secretion from the splenocytes compared to the THR group. The AMX group had decreased IL-10 secretion from the PBLs and IL-4 secretion from the splenocytes. In summary, the results suggest that supplementation of arginine and glutamine in the resuscitation fluid may alleviate splenocytic proinflammatory response and that of glutamine and amino acid mixture may elevate phagocytosis and modulate the immunity of T-helper cells in trauma-hemorrhagic shock.
Funding Source: NSC 102-2320-B-030-005-MY3
Fu Jen Catholic University
new Taipei City, Taipei, Taiwan (Republic of China)