Poster Topical Area: Dietary Bioactive Components

Location: Hall D

Poster Board Number: 319

P08-061 - Hypocholesterolemic Effect of Nanoemulsion Quercetin in Rats Fed High-cholesterol Diet

Monday, Jun 11
8:00 AM – 3:00 PM

Objectives: This study aimed at investigating the hypocholesterolemic effect of quercetin and nanoemulsion quercetin in rats fed high-cholesterol diet.

Male, Sprague-Dawley rats (6-week-old) were randomly divided into 6 groups as follows: a normal diet (NOR), high-cholesterol diet (HC), and HC containing 0.05% quercetin (LQ), 0.1% quercetin (HQ), 0.05% nanoemulsion quercetin (LNQ) or 0.1% nanoemulsion quercetin (HNQ). All groups of rats were fed each experimental diet for 4 weeks.

At the end of the experiment, serum levels of total cholesterol (TC) and low-density lipoprotein cholesterol (LDL-C) were lower in the HNQ group in comparison with the HC group(P<0.05). The hepatic level of TC was lower in the LNQ group compared to the HC group(P<0.05). The fecal excretion of triglycerides (TG), TC and total bile acids (TBA) were higher in the nanoemulsion quercetin supplemented groups compared to the HC group(P<0.05), whereas quercetin did not. In addition, the mRNA expression of genes involved in hepatic bile acid synthesis and cholesterol efflux, such as Liver X receptor α (LXRα), ATP binding cassette subfamily G member 5 (ABCG5), ATP binding cassette subfamily G member 8 (ABCG8), and cholesterol 7 alpha-hydroxylase (CYP7A1), was up-regulated in the liver of rats supplemented with nanoemulsion quercetin compared to the HC group (P<0.05), whereas quercetin did not. Further, the activity of CYP7A1 in liver was enhanced by the nanoemulsion quercetin supplementation(P<0.05), whereas quercetin did not.

These results suggest that nanoemulsion quercetin would have beneficial effects on hypercholesterolemia in vivo possibly through synthesis of hepatic bile acid and excretion of cholesterol.

Funding Source: This study was supported by a grant from the Ottogi Foundation in Korea

CoAuthors: Hye-yeon Son – Ewha Womans university; Mak-soon Lee – Ewha Womans university; Eugene Chang – Ewha womans university; Seog-Young Kim – Ewha womans university; Hyunmi Ko – Ewha womans universiy; Chong-Tai kim – Korea Food Research Institute; Yangha Kim – Ewha Womans university

Soojin Lee

Ewha womans university
Seoul, Seoul-t'ukpyolsi, Republic of Korea