Poster Topical Area: Dietary Bioactive Components
Location: Hall D
Poster Board Number: 319
Objectives: This study aimed at investigating the hypocholesterolemic effect of quercetin and nanoemulsion quercetin in rats fed high-cholesterol diet.
Methods: Male, Sprague-Dawley rats (6-week-old) were randomly divided into 6 groups as follows: a normal diet (NOR), high-cholesterol diet (HC), and HC containing 0.05% quercetin (LQ), 0.1% quercetin (HQ), 0.05% nanoemulsion quercetin (LNQ) or 0.1% nanoemulsion quercetin (HNQ). All groups of rats were fed each experimental diet for 4 weeks.
Results: At the end of the experiment, serum levels of total cholesterol (TC) and low-density lipoprotein cholesterol (LDL-C) were lower in the HNQ group in comparison with the HC group(P<0.05). The hepatic level of TC was lower in the LNQ group compared to the HC group(P<0.05). The fecal excretion of triglycerides (TG), TC and total bile acids (TBA) were higher in the nanoemulsion quercetin supplemented groups compared to the HC group(P<0.05), whereas quercetin did not. In addition, the mRNA expression of genes involved in hepatic bile acid synthesis and cholesterol efflux, such as Liver X receptor α (LXRα), ATP binding cassette subfamily G member 5 (ABCG5), ATP binding cassette subfamily G member 8 (ABCG8), and cholesterol 7 alpha-hydroxylase (CYP7A1), was up-regulated in the liver of rats supplemented with nanoemulsion quercetin compared to the HC group (P<0.05), whereas quercetin did not. Further, the activity of CYP7A1 in liver was enhanced by the nanoemulsion quercetin supplementation(P<0.05), whereas quercetin did not.
Conclusions: These results suggest that nanoemulsion quercetin would have beneficial effects on hypercholesterolemia in vivo possibly through synthesis of hepatic bile acid and excretion of cholesterol.
Ewha womans university
Seoul, Seoul-t'ukpyolsi, Republic of Korea