Poster Topical Area: Nutritional Microbiology

Location: Auditorium

Poster Board Number: 240

P22-011 - Altered gut microbiota in preterm newborns with necrotizing enterocolitis using High-Throughput sequencing

Monday, Jun 11
8:00 AM – 3:00 PM

OBJECTIVES: Recent studies have shown that pathophysiology of necrotizing enterocolitis (NEC) includes intestinal microbial dysbiosis and mucosal barrier disruption. This study tends to investigate intestinal microbiota in preterm newborns with NEC.
METHODS: Our prospective study enrolled 24 preterm newborns admitted to the NICU in Shanghai Children’s Medical Center from March 2013 to August 2014, whose gestational age ranged from 29 to 33 weeks. Among the 24 preterm, 4 were diagnosed as NEC, while 3 were treated with antibiotics due to serious infections (infection group) and 17 without any infectious complications (normal group). Totally 192 longitudinal fecal samples were collected right from admission until discharge day. The Intestinal microbiota composition and its longitudinal trend were analyzed using Illumina-MiSeq high-throughput sequencing.
RESULTS: At phylum level, Firmutes and Proteobacteria dominated respectively in three groups, while Proteobacteria abundance of NEC group significantly ranked first (NEC vs Infection vs Normal: 59.84% vs 42.97% vs 44.13%,  p=0.048). At class level, three groups shared the same domination microorganisms which are Bacilli, Clostridia and Gammaproteobacteria, and Gammaproteobacteriae abundance of NEC group is significantly the highest (NEC vs Infection vs Normal: 53.63% vs 33.96% vs 39.46, p=0.018). Longitudinal comparison showed different microbial colonization pattern among three groups. Notwithstanding the same microbial development mode shared by three groups from Bacilli to Gammaproteobacteriawithin two weeks after birth, Bacilli and Clostridia domination from the 14th to 30th day of life and Clostridia domination after the 30th day of life in NEC group was distinctive and noticeable.
CONCLUSIONS: Abnormal intestinal microbiota at early life might account for NEC. However, more longitudinal studies with larger sample sizes are needed to reveal microbial dysbiosis at different stages before and after the onset of NEC, hopefully to provide evidence for its early recognition and prevention.


CoAuthors: JIAYI LIU – shanghai childrens medical center

Li Hong

Professor
Shanghai Childrens Medical Center
Shanghai, Shanghai, China (People's Republic)