Poster Topical Area: Energy and Macronutrient Metabolism
Location: Hall D
Poster Board Number: 501
Objective: Grain sorghum (GS) lipid extract has shown protection against high cholesterol in both plasma and liver and, to a lesser degree, remediation in the gut microbiota in response to an atherogenic diet. However, the impact of GS lipids on cellular stress induced by such a diet remains largely unknown. The objective of this research was to determine the ability of GS crude lipid (GS-CL) obtained from the surface of GS whole kernel to protect energy metabolism and short chain fatty acid (SCFA) profile produced by the gut microbiome that may be negatively affected by an atherogenic diet.
Methods: Male hamsters were fed with either a low-fat control diet, an atherogenic diet or an atherogenic diet supplemented with 1, 3, 5% (w/w) GS-CL for four weeks. Colon tissue was collected after euthanasia and analyzed by targeted metabolomics.
Results: Analysis showed that the atherogenic diet disrupted cellular redox balance and impaired energy generation, promoting gluconeogenesis and anaplerosis from pyruvate and amino acids. The GS-CL supplements were able to maintain energy levels, mitigated the accumulation of glycolysis and TCA intermediates, but were not able to rebalance redox equilibrium completely. Moreover, multivariant analysis including orthogonal-partial least squares-discrimination analysis (OPLS-DA) demonstrated that the GS-CL supplements were able to gradually shift the atherogenic affected metabolomics to levels trending or comparable to the control level. In addition, the GS-CL supplements positively affected the SCFA produced by colon microbiome against atherogenic diet induced changes.
Conclusions: This study provides whole-scale impact of GS-CL on cellular energy and central carbon metabolism using a combined approach of metabolomics and multivariant analysis, which effectively demonstrate the potentials of GS-CL as a supplement to prevent or contain chronic metabolic diseases.
PhD graduate student
University of Nebraska-Lincoln