Poster Topical Area: Dietary Bioactive Components
Location: Hall D
Poster Board Number: 351
Background: DNA methylation affects gene expression by changing the level of transcription and mRNA synthesis. DNA methyltransferases (DNMTs) are responsible for the methylation of DNAs, and hypermethylation of DNAs by DNMTs may affect physiological functions, thus leading to chronic diseases. Oxidative damages to DNAs or cells that caused by reactive oxygen species (ROS) also affect physiological functions. Additionally, ROS increases DNMTs activities, thus leading to hypermethylation of DNAs. Therefore, neutralization of ROS by antioxidants is important to prevent oxidative damages and hypermethylation of DNAs. Objective: This study was conducted to the measure effects of antioxidant treatments on DNMT gene expression.
Methods: Murine adipocytes (1x107) were treated with myricetin, resveratrol, lipoic acid, α-tocopherol (0, 10, 50, 100 µM), or superoxide dismutase (100 U/mL, control antioxidant), and then treated with superoxide anion. After superoxide anion treatment, total RNA was extracted and purified using an RNeasy Mini kit. DNMT1, DNMT3a, and DNMT3b gene expression by real-time PCR was measured. The fold change in expression of the DNMTs was calculated using the 2-ΔΔCT method.
Results: Resveratrol or myricetin (100 µM) treatment significantly downregulated DNMT1 and DNMT3a expression, but did not affect DNMT3b expression. Lipoic acid or α-tocopherol treatment did not affect DNMT1, DNMT3a, or DNMT3b expression.
Conclusions: Further study is needed to determine if resveratrol or myricetin also affects DNMTs expression in adipocytes treated with different reactive oxygen species, and to determine how resveratrol or myricetin downregulated DNMTs expression in superoxide anion treated murine adipocytes.
North Dakota State University
Fargo, North Dakota