Poster Topical Area: Energy and Macronutrient Metabolism

Location: Hall D

Poster Board Number: 445

P10-045 - Gene Expression in Differentiated 3T3-L1 Cells: Effect of Ginger Extract

Monday, Jun 11
8:00 AM – 3:00 PM

Ginger have gained importance recently for the treatment of obesity and its complications The gingerols and shogaols in the ginger extract (GE) may have thermogenic activity. The objective of this study was to assess in vitro and in vivo the effects of SCE GE on adipogenesis and thermogenesis. Obesity is the result of increased adipogenesis and down regulation of lipolysis. The differentiation of pre-adipocytes into mature adipocytes, is regulated by different transcription factors such as peroxisome proliferator-activated receptors (PPARs) and CCAAT/enhancer binding proteins (C/EBPs). We investigated the effects of ginger extract on lipogenesis and thermogenic gene expression using in vitro 3T3-L1 mouse cell model. After 6 days of treatment with GE (50µg/ml) during adipocyte differentiation, 3T3-L1 cells lysates were harvested and real-time PCR was performed to evaluate the expression of the adipogenic and thermogenic marker genes Among the genes selected, C/EBP alpha and PPAR gamma are the main regulators of adipogenesis and key transcription factors that are responsible for the activation of adipogenic genes. Acetyl-CoA carboxylase alpha (ACCA), Fatty Acid Synthase (FASN) and Stearoyl-CoA Desaturase 1 (SCD-1) are involved in lipogenic process, DLK1 is a negative regulator that inhibits adipogenesis, PGC1 alpha, SIRT1 and UCP1 are involved in thermogenesis. All statistics were analyzed using GraphPad Prism. Data were expressed as mean values ± SEMs. Results were considered significant at p < 0.05. Our results showed that GE treatment significantly down regulated ACCA, FASN, SCD1, PPAR gamma and CEBPA gene expression and slightly upregulated PGC1 alpha expression, compared to control. FASN gene regulate fatty acid synthesis and lipid accumulation in liver and adipose tissue. SCD1 genes catalyze the conversion of saturated fatty acids (FAs) into monounsaturated fatty acids. PGC1 alpha genes are related to the process of thermogenesis. These results suggest that GE treatment promote high expression of these genes and help consuming energy excess as heat and decreasing the storage of energy excess as fat. The underlying molecular mechanism for this reduction were related to the inhibition of adipogenesis and an upregulation of thermogenesis.




Funding Source: OmniActive Health Technologies Ltd., India

CoAuthors: Xin Yi.., Ph.D – OmniActive Health Technologies Ltd.; Jayant Deshpande, Ph.D – OmniActive Health Technologies Ltd.; Vijaya Juturu, Ph.D – OmniActive Health Technologies Inc.

Khadija Ghanam

Clinical Nutritional Biochemist
Omniactive Health Technologies (canada) Limited
Charlottetown, Prince Edward Island, Canada