Poster Topical Area: Energy and Macronutrient Metabolism

Location: Hall D

Poster Board Number: 492

P10-093 - Branched chain amino acids and lipid profile among Normal weight adults with Impaired Glucose Tolerance

Monday, Jun 11
8:00 AM – 3:00 PM

Objectives
This study explored the relationship between serum branched-chain amino acids (BCAA) and lipid profiles among normal-weight adults with impaired glucose tolerance (IGT).

Methods
Demography, anthropometry and blood pressure (BP) measurements, fasting and 2h blood samples were obtained from 15 newly onset IGT and 15 normoglycemic subjects [(matched for age, sex and body mass index(BMI)] using standardized methods. Fasting blood glucose (FBG), 2h-plasma glucose (2h-PG), fasting insulin (FI), total cholesterol (TC), triglycerides (TG), high-density lipoprotein cholesterol (HDL-c) and low-density lipoprotein (LDL-c) were determined using auto-analyzer with commercial kits and BCAA [isoleucine, leucine and valine] were profiled using tandem mass spectrometry. χ² test or t-test for categorical (%) or continuous variables (means±SD) respectively was applied to compare differences between normal weight without IGT (NW) and normal weight with IGT (NWI) groups, Pearson's correlation (r) was conducted to evaluate the relationship between BCAA, lipid profiles FBG, 2h-PG, FI and two-sided P < 0.05 was considered statistically significant.


Results
Age, waist circumference, systolic BP and BMI tended significantly higher among NWI. Similarly, FBG and 2h-PG were significantly (P < 0.01) higher among NWI (5.2±1.3mmol/L and 10.4±4.5mmol/L respectively) compared to NW (3.9±0.3mmol/L and 5.4±1.0mmol/L respectively) but FI differed insignificantly. Though serum LDL-c tended to be higher among NWI (3.4±0.8mmol/L) compared to NW (2.8±0.8mmol/L); TC, TG, HDL-c and BCAA profiles were insignificantly different between the two groups. Correlation coefficients between FBG, 2h-PG, FI and BCAA were insignificantly different (even after stratifying IGT status). However, HDL-c negatively correlated with Ile (r = - 0.37, P < 0.05) and Leu (r = - 0.37, P < 0.05).


Conclusion
Higher BCAA negatively alters HDL-c profile.




Funding Source: China Scholarship Council (2015BSZ778)
National 12th Five-Year Scientific and Technical Support Program of China (2012BAI02B02)

CoAuthors: Liyan Liu – Harbin Medical University; Rennan Feng – Harbin Medical University; Ying Li – Harbin Medical University; ChangHao Sun – Harbin Medical University

Akinkunmmi PAUL.. Okekunle

Graduate Student
Harbin Medical University
Harbin, Heilongjiang, China (People's Republic)