Innovation Showcase

Innovation Showcase Thursday morning



iPSC technology enabled in vitro studies with human neurons for investigating disease mechanisms and for finding treatments.

We will present techniques to dissect the functional phenotype of A53T α-synuclein dopaminergic neurons, modeling Parkinson’s disease. We will discuss how the electrical activity of whole cell networks can be monitored label-free and long-term, at high spatio-temporal resolution. By observing cell networks across multiple days, the capability of iPSC-derived neurons to form synaptic connections can be analyzed. In addition, we will show how the activity of single neurons can be isolated and studied, together with subcellular details, such as the propagation of action potentials along single axons.

Our methods are powered by a high-throughput electrophysiology platform using high-resolution microelectrode array (MEA) technology. The platform allows access to individual cells simultaneously through 26’400 electrodes. With this platform, we found differences in physiological activity between the A53T α-synuclein cell line and the isogenic control cell line. Phenotype differences were detected at different scales, ranging from network connectivity to subcellular structures as axons.

Innovation Showcase: Maxwell Biosystems

6/21/2018
08:00 - 08:30

Presented by: Maxwell Biosystems 

Presentations:

Michele Fiscella

ETH Zurich / MaxWell Biosystems AG, Basel-Stadt, Switzerland

Dr. Michele Fiscella obtained the MSc in Biotechnology at the University of Bologna (Italy) in 2008. After university, Dr. Fiscella joined the Swiss interdisciplinary PhD program at the laboratories of Prof. Dr. Hierlemann at ETH Zurich and Prof. Dr. Roska at Friedrich Miescher Institute (FMI) in Basel (Switzerland). PhD work focused on high-throughput electrophysiology recordings by microelectrode arrays (MEAs) and analysis/modeling of neural signals. After obtaining the PhD, Dr. Fiscella worked with transgenic animals modeling human diseases. In September 2016, Dr. Fiscella co-founded MaxWell Biosystems AG, a Swiss based company commercializing high-resolution MEAs. Dr. Fiscella is currently working as VP Scientific Affairs at MaxWell Biosystems AG. Dr. Fiscella's activities focus on developing novel in vitro electrophysiology assays, using iPSC-derived neurons modeling human diseases.

Presentation(s):

Send Email for Michele Fiscella


Assets

Innovation Showcase: NOVEL FUNCTIONAL ANALYSIS TECHNIQUES FOR ACCURATE PHENOTYPE CHARACTERIZATION OF HUMAN IPSC-DERIVED NEURONS - Maxwell Biosystems



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Send Email for Innovation Showcase: NOVEL FUNCTIONAL ANALYSIS TECHNIQUES FOR ACCURATE PHENOTYPE CHARACTERIZATION OF HUMAN IPSC-DERIVED NEURONS - Maxwell Biosystems