Innovation Showcase Friday morning

Innovation Showcase

Innovation Showcase: CELL SURFACE MARKER SCREENING FOR THE ISOLATION OF NEURAL CELL SUBSETS - BD Biosciences

6/22/2018
08:00 - 08:30

Understanding the underlying molecular and biochemical characteristics of stem cell differentiation is one of the most significant challenges facing stem cell biologist today. Central to solving these complex questions is learning how to better characterize, both phenotypically and functionally, as well as identify and isolate specific cells throughout stem cell differentiation. During the last several years, life science research tools companies have continued to refine new approaches to help facilitate discovery in this area of biology. This presentation will focus on Identifying cellular immune-phenotype to enrich neurons from H9 neural induction cultures using a cell surface marker screen of the SFEB neural induction cultures. Data will be presented Heat map of the cell surface markers screened with markers that were preferentially expressed on neurons. The markers that were identified as preferentially expressed on neurons were CD7, CD24, CD56, CD57 ,CD171 and CD200, as compared to the markers identified to be expressed on non-neuronal cells which were CD49f, CD151, CD184, CD340, b2-ugloblin, HLA-ABC, and SSEA-1. The markers that showed the highest differential expression were then co-stained to see what combination of markers would identify a population of cells that could be sorted and analyzed. Today, with the use of flow cytometry, large-scale, systems biology biomarker screening applications have been facilitated by the use of high-throughput techniques, high complexity flow cytometry analysis and other novel tools for analyzing the heterogeneity of stem cell differentiation.

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Innovation Showcase: CELL SURFACE MARKER SCREENING FOR THE ISOLATION OF NEURAL CELL SUBSETS - BD Biosciences



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