Stem Cell Niches

Poster

F-1115 - DIFFERENTIATION OF HUMAN EMBRYONIC STEM CELLS TO FEMALE GERM CELLS USING HUMAN CUMULUS CELLS CONDITIONED MEDIUM

6/22/2018
18:00 - 19:00

Human embryonic stem cells (hESCs) are pluripotent stem cells that can differentiate to three germ layers derivatives and germ cells. Because of ethical and technical problems, it is difficult to study early germ cell development in human. In vitro differentiation of pluripotent stem cells to germ cells is a proper way to help to infertile couples and as a model for scientific researches. Cumulus cells are nursing cells that support oocyte during development by secretion of some factors like growth factors. Conditioned medium from cumulus cells may contain this factors that can effect on differentiation of hESC to female germ cells. Embryoid bodies (EBs) from Yazd4 (female hESC line) were divided into 2 groups: a) spontaneously differentiation; and b) treated with human cumulus cells conditioned medium. EBs from both groups were cultured for 14 days. Pluripotency and germ cells genes expression were examined by Q-PCR at the time of days 0, 4, 7 and 14. Q-PCR results showed that in both groups NANOG expression decreased from day 0 to day 14. Unlike NANOG, VASA expression increased from day 0 to day 14. VASA expression in group a was more than group b. Unlike VASA, NANOG was expressed more in group b. Further works with more markers and techniques are in progress to be able to come out with a precise interpretation. So far, our data confirm the previously reported studies regarding the ability of hESCs to form germ cells in both spontaneously and cumulus cell conditioned medium cultures.


 

Somayyeh Sadat Tahajjodi

Stem Cell Biology Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

Presentation(s):

    Send Email for Somayyeh Sadat Tahajjodi


    Assets

    F-1115 - DIFFERENTIATION OF HUMAN EMBRYONIC STEM CELLS TO FEMALE GERM CELLS USING HUMAN CUMULUS CELLS CONDITIONED MEDIUM



    Attendees who have favorited this

    Please enter your access key

    The asset you are trying to access is locked. Please enter your access key to unlock.

    Send Email for F-1115 - DIFFERENTIATION OF HUMAN EMBRYONIC STEM CELLS TO FEMALE GERM CELLS USING HUMAN CUMULUS CELLS CONDITIONED MEDIUM