Neural Development and Regeneration

Poster

F-2025 - COAXING OF HUMAN MESENCHYMAL STEM CELLS INTO DOPAMINERGIC NEURONAL CELLS IN VITRO: A POTENTIAL THERAPEUTIC APPROACH FOR MEDICAMENT OF PARKINSONISM

6/22/2018
18:00 - 19:00

Parkinson’s disease (PD) is a neurodegenerative movement disorder, characterized by a loss of midbrain dopaminergic neurons. Pharmacological management of the disease with L-DOPA is helpful only at initial stages; hence, it further requires the use of cell based treatment to manage the disease in a better and efficient manner. Considering this point, several stem cell researchers have explored the neurogenic potential of Mesenchymal Stem Cells (MSCs) and their effects in improving the behavioural functions in PD rat model. MSCs from various tissue sources like bone marrow, adipose tissue, dental pulp, Wharton’s jelly, etc. have been explored for their neurogenic potential under both in vitro and in vivo conditions. However, bone marrow derived Mesenchymal Stem Cells have been highly explored stem cell candidates in this regard. Hence, the current study was planned to explore a) The functional behaviour of differentiated BM-MSCs using FGF2 and FGF2+22-hydroxycholesterol (HC), and b) The difference in the neurogenic potential of BM-MSCs in naive (N=03) and differentiated state (N=03 for each group) up on transplantation in Parkinson’s disease Wistar rat model. PD rat model was prepared by creating unilateral lesion by injecting 6-OHDA stereotaxically in the substantia nigra pas compacta region of the midbrain. Established PD rat models, after their behavioural assessment (Rotarod and beam tests), were considered for further studies. Post characterization of the differentiated BM-MSCs, they were transplanted in the brain at the same coordinates as the lesion. Neuroregenerative effect of the transplanted BM-MSCs was assessed 4 weeks after transplantation on the basis of behavioural changes, H & E staining and immunohistochemistry analysis for the expression of MAP2 and TH proteins. To ensure the homing of the transplanted cells, FISH was performed with human X- probes. Transplanted cells showed no inflammatory symptoms in the rats, also confirmed by H & E staining. It was observed that BM-MSCs which were pre- primed into cells of dopaminergic neuronal lineage had better neuroregenerative effect after transplantation into PD rat models. With the current study, it may also be concluded that BM-MSCs primed with FGF2+22-HC had better neuroregenerative potential as compared to those primed with FGF2 alone.


 

Manisha Singh

All India Institute of Medical Sciences, Delhi, India

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    F-2025 - COAXING OF HUMAN MESENCHYMAL STEM CELLS INTO DOPAMINERGIC NEURONAL CELLS IN VITRO: A POTENTIAL THERAPEUTIC APPROACH FOR MEDICAMENT OF PARKINSONISM



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    Send Email for F-2025 - COAXING OF HUMAN MESENCHYMAL STEM CELLS INTO DOPAMINERGIC NEURONAL CELLS IN VITRO: A POTENTIAL THERAPEUTIC APPROACH FOR MEDICAMENT OF PARKINSONISM