Abiotic Stress/Whole Plant Bio

Abstract

CS-9-5 - QTLs Maintaining Pollen Fertility under Salt Stress Detected by Exome QTL-seq and Interval Mapping in Barley

Sunday, July 15
4:38 PM - 4:58 PM

Soil salinity is one of the most serious problems limiting crop production in world’s dry areas. Enhancing salt stress tolerance is one of the most important strategies for increasing global food production. Barley (Hordeum vulgare L.) is known as the salt-tolerant crop and salt tolerance differs among cultivars. We previously found that long-term salinity stress significantly reduced grain fertility in the salt-sensitive barley accession, OUC613, but not in the salt-tolerant accession, OUE812, resulting in large differences in grain yield. Here, we examined the underlying causes of the difference in grain fertility between these accessions under long-term treatment with 150 or 200 mM NaCl from the seedling stage to harvest and identified quantitative trait loci (QTLs) for maintaining grain fertility under salt stress. In an artificial pollination experiment of the two accessions, grain fertility was significantly reduced only in OUC613 plants produced using pollen from plants grown under 150 mM NaCl stress. By contrast, grain fertility was not significantly decreased in OUE812 among plants from crosses between female and male plants grown under any conditions. These results suggest that the low grain fertility of OUC613 under salts stress was mainly due to reduced pollen fertility. Using newly developed technique, QTL-seq combined with exome-capture sequencing (exome QTL-seq) and conventional composite interval mapping of recombinant inbred lines derived from a cross between OUE812 and OUC613, we identified a QTL for grain fertility on chromosome 2H. The allele of OUE812 maintained grain fertility under the NaCl stresses. The QTL region includes two genes encoding an F-box protein and a TIFY protein that are associated with male sterility. The QTL identified on chromosome 2H is associated with maintaining pollen fertility under salt stress, highlighting the importance of this region for maintaining grain fertility under salt stress.

Asuka Kodama

Ph. D Student
Graduate School of Agriculture, Tokyo University of Agriculture and Technology

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CS-9-5 - QTLs Maintaining Pollen Fertility under Salt Stress Detected by Exome QTL-seq and Interval Mapping in Barley



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