Genetics/Genomics

Abstract

CS-12-5 - An inhibition of RHD3 fusion activity by Lunapark is essential for generation of interconnected tubular ER network

Monday, July 16
2:23 PM - 2:43 PM

The formation of the interconnected tubular ER network requires homotypic fusion, which is mediated by Atlastin large GTPases, including RHD3 (ROOT HAIR DEFECTIVE 3) in Arabidopsis. To generate an interconnected ER network, the fusion activity of Atlastin proteins must be regulated. Such a regulation is, however, not well studied. We showed here that RHD3 physically interacts with two Arabidopsis Lunapark proteins (LNPs) LNP1 and LNP2 at 3-way junctions of the ER. We found that, recruited by RHD3 to newly formed 3-way junctions, LNPs stabilize the nascent 3-way junctions of the ER by inhibiting the fusion activity of RHD3. In Arabidopsis lnp mutant cells, the ER is sheet-like with dense 3-way junctions, which is linked with higher RHD3 protein level than that in wild type plants. We proposed that, after the ER fusion is completed, RHD3 is promoted to be degraded by LNPs so nascent 3-way junctions can be stabilized.

Co-Authors

Huanquan Zheng, Ph.D – associate professor, Biology department, McGill University

Jiaqi Sun

Ph.D student
McGill University

Jiaqi Sun, Ph.D student, Biololy Department, McGill University

Presentation(s):

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CS-12-5 - An inhibition of RHD3 fusion activity by Lunapark is essential for generation of interconnected tubular ER network



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