Cell/Development/Systems

Abstract

CS -15-2 - The maize OPAQUE1 myosin XI is required for asymmetric cell division during stomatal development

Monday, July 16
1:23 PM - 1:43 PM

The arrangement and identity of cells within a tissue is set up early in development. Asymmetric cell divisions determine cellular patterning by determining the relative placement of various cell types. The stereotypic divisions that form stomata have been a model for studying asymmetric division in both monocots and dicots. Monocot grass stomata are made of 4 cells: 2 guard cells flanked by a pair of subsidiary cells. The asymmetric division of a subsidiary mother cell (SMC) yields a subsidiary cell and a pavement cell, and is a useful cellular model for determining the molecular determinants that orchestrate asymmetric divisions. Based on the pattern of division, it is thought that the SMC responds to an extrinsic cue sent from the adjacent guard mother cell (GMC) which promotes the asymmetric division of the SMC. Asymmetric division occurs in three phases: (I) polarization, (II) division plane establishment and maintenance and (III) mitosis & cytokinesis. Multicellular plant myosins are divided into 2 classes: class VII myosins, thought to be involved in cytokinesis, and class XI, thought to be involved in trafficking of vesicles and organelles. We hypothesized the class XI myosin, OPAQUE1 (O1) would play a role in cell polarization by promoting polarization of molecular determinants that promote polarization, and/or driving nuclear polarization. o1 mutants show patterning defects due to aberrant SMC asymmetric divisions. However, polarization of SMCs is normal, indicating a role for O1 post-polarization. Localization data indicate O1 associates with cytokinetic structures. Analysis of cytoskeletal elements in o1 mutants confirms a role for O1 post-polarization, suggesting that class XI myosins also play a role during mitosis.


 

Co-Authors

Janette Mendoza – University of New Mexico; Liang Hong – University of California, Riverside; Carolyn Rasmussen – University of California, Riverside

Michelle Facette

Assistant Professor
University of New Mexico

Presentation(s):

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CS -15-2 - The maize OPAQUE1 myosin XI is required for asymmetric cell division during stomatal development



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