Biotic Stress/Applied Plant Bio

Abstract

CS-13- 2 - Phosphocode-dependent functional dichotomy of the common co-receptor BAK1 in plant signaling

Monday, July 16
1:23 PM - 1:43 PM

Plants rely on cell surface-localized receptor kinases for their survival. Plant receptor kinases form ligand-induced complexes with shape-complementary co-receptors, which are required for their activation. The best-characterized plant co-receptor is BAK1 (also called SERK3), which associates with numerous leucine-rich repeat receptor kinases to control both immunity and several aspects of growth and development. However, the mechanisms by which BAK1 controls the activation of these receptors remain mostly unknown. We have identified key regulatory phosphorylation events controlling the functionality of BAK1 and more generally leucine-rich repeat receptor kinases. Through a combination of phospho-proteomics and targeted mutagenesis, we identified conserved phosphosites that are required for BAK1 immune function in Arabidopsis thaliana. The inability to phosphorylate on these residues abolishes cellular immune outputs induced by bacterial pathogen-associated molecular patterns, and leads to impaired anti-bacterial immunity. Strikingly, these phosphosites are not required for BAK1/SERK-dependent brassinosteroid-regulated signaling. In addition to revealing a critical role for BAK1 C-terminal tail phosphorylation, we also identified a conserved phosphorylated tyrosine residue required for the functionality of the majority of Arabidopsis leucine-rich repeat receptor kinases, and which separates them into two distinct functional classes. Our results thus highlight a phosphocode-based dichotomy of BAK1 functionality in plant signaling, and provide novel insights into receptor kinase activation, which have broad implications for our understanding of how plants grow, develop and respond to their changing environment.


 

Co-Authors

Artemis Perraki – The Sainsbury Laboratory, Norwich, UK; Thomas DeFalco – The Sainsbury Laboratory, Norwich, UK; Paul Derbyshire – The Sainsbury Laboratory, Norwich, UK; Julian Avila – Howard Hughes Medical Institute, University of Washington, Seattle, USA & Department of Biology, University of Washington, Seattle, USA; David Séré – The Sainsbury Laboratory, Norwich, UK; Jan Sklenar – The Sainsbury Laboratory, Norwich, UK; Xingyun Qi – Howard Hughes Medical Institute, University of Washington, Seattle, USA & Department of Biology, University of Washington, Seattle, USA; Lena Stransfeld – The Sainsbury Laboratory, Norwich, UK; Benjamin Schwessinger – The Sainsbury Laboratory, Norwich, UK; Yasuhiro Kadota – The Sainsbury Laboratory, Norwich, UK; Alberto Macho – The Sainsbury Laboratory, Norwich, UK; Shushu Jiang – The Sainsbury Laboratory, Norwich, UK; Daniel Couto – The Sainsbury Laboratory, Norwich, UK; Keiko Torii – Howard Hughes Medical Institute, University of Washington, Seattle, USA & Department of Biology, University of Washington, Seattle, USA; Frank Menke – The Sainsbury Laboratory, Norwich, UK; Cyril Zipfel – The Sainsbury Laboratory, Norwich, UK

Thomas A. DeFalco, PhD

Postdoctoral Scientist
University of Zurich

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CS-13- 2 - Phosphocode-dependent functional dichotomy of the common co-receptor BAK1 in plant signaling



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