Genetics/Genomics

Abstract

CS-7-6 - Histone H1 mutation affects genomic imprinting and DNA methylation in Arabidopsis

Sunday, July 15
4:58 PM - 5:00 PM

Genomic imprinting, the differential expression of parental alleles, regulates development in mammals and plants. DNA methylation is regarded as a main cause of imprinting by silencing one parental allele. It has been shown that DEMETER (DME), a DNA glycosylase, demethylates DNA and impacts genomic imprinting in Arabidopsis. It is also known that histone H1 variants (H1.1, H1.2, and H1.3) interact with DME in vitro and regulate the expression of imprinted genes in the Arabidopsis endosperm. Based on expression of the transgene H1 promoter:GUS, three histone H1 genes are widely expressed in different tissues, especially in embryo, endosperm, root tips and floral organs. Each H1 gene has its own expression pattern. H1.2 promoter:GFP reporter protein is expressed in the central cell and endosperm. Yeast two-hybrid assays reveal that histone H1.2 binds strongly with DME N-terminal region compared with DME C-terminal region which contains three domains (A, Glycosylase, and B domains). In addition, histone h1 triple mutant has reduced MEDEA:GFP and FWA:GFP expression in the central cell and endosperm. Furthermore, the DNA methylome data of embryo and endosperm of histone h1 triple mutant reveal that the maternal h1 mutant allele affects the methylation level of most DME targets. These results suggest that histone H1 is involved in DME-mediated global DNA demethylation in Arabidopsis.


 

Co-Authors

Arthur Bartels – Saint Louis University; Tzung-Fu Hsieh – North Carolina State University; Wenyan Xiao – Saint Louis University

Qiang Han

PhD student
Saint Louis University

Presentation(s):

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CS-7-6 - Histone H1 mutation affects genomic imprinting and DNA methylation in Arabidopsis



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