Genetics/Genomics

Abstract

CS-22-1 - Global analysis of small RNAs and their targets for regulatory network controlling in floral symmetry in Sinningia speciosa

Tuesday, July 17
8:33 AM - 8:53 AM

Floral zygomorphic symmetry has evolved multiple times during flowering plants diversification, which contributes to the evolution of specialized pollination syndrome thus enhanced species richness. The key gene CYCLOIDEA is well understood in developing zygomorphy in angiosperm, however, its regulating and downstream genetic network remain largely unknown. Most Gesneriaceae species bear zygomorphic flowers, dorsi-ventral petal asymmetry can be distinguished by the size and color of the petals. Comparison of gene expression profiles between petals from dorsal and ventral parts of the flower should help to identify key genes responsible for zygomorphy. MicroRNAs (miRNAs) are key regulators directing leaf polarity and flower development. Here, we profiled and compared the small RNAs expressed in dorsal and ventral petal of S. speciosa. Among 157 unique miRNA sequences we identified, 56 miRNAs are conserved in flowering plants, while the other 101 are specific to S. speciosa. A combination of computational and degradome analysis allowed us to obtained 109 statistically significant targets. Statistical analysis revealed 8 differentially expressed miRNAs between dorsal and ventral petals. Notably, among them, miR390 is known to regulate leaf polarity by triggering trans-acting siRNA production from TAS3 to control auxin response. Furthermore, the fragment of ARF3 (Auxin response factor) had been found in our degradome data. The last but not the least, loads of auxin-inducible genes, the downstream targets of ARF3, like IAA14, AUX26 and XTH33, show differential expression in dorsal and ventral petal in our transcriptome-seq data. By integrating small RNA, degradome and transciptome data, we discover the presence of miR390-TAS3-tasiARF-ARF3-auxin responsive genes regulatory circuit in zygomorphic flowers of S. speciosa. This finding implies a co-option in development of leaf adaxial-abaxial polarity into floral dorsi-ventral zygomorphy. 


 

Co-Authors

Wang Chun-Neng – National Taiwan University; Chen Ho-Ming – Academia Sinica ; Hou Bo-Han – Academia Sinica

Nien Ya-chi, MA

Master student
National Taiwan University

Presentation(s):

Send Email for Nien Ya-chi


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CS-22-1 - Global analysis of small RNAs and their targets for regulatory network controlling in floral symmetry in Sinningia speciosa



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