Biochemistry

Abstract

CS-11-2 - Evolutionary innovations enhance metabolic diversity: biosynthesis of acylglucoses in Solanum pennellii trichomes

Monday, July 16
1:23 PM - 1:43 PM

We study secretory glandular trichomes of species in the Solanaceae family to understand the emergence of metabolic pathway innovations. Acylsugars are specialized metabolites produced in the apical cells of specific trichome types in this family. These compounds are of fundamental interest due to their diverse structures, and are of practical importance in protection from insects and microbial pathogens. Acylsugars consist of two components: the central sugar core, primarily sucrose or glucose, and peripheral decorations of esterified fatty acid acyl chains ranging from C2 to C12 with varied branching patterns and at different positions on the core. We have extensively studied acylsucrose biosynthesis in cultivated tomato; however, there are many species that produce uncharacterized structurally diverse acylsugars. For example, Solanum pennellii is a wild relative of cultivated tomato that is studied due to its broad resistance to insects and herbivores. S. pennellii LA0716, an accession found in the southern Atacama desert, produces unusually large amounts of acylsugars – consisting primarily of acylglucoses. We are studying the biosynthesis of acylglucoses in S. pennellii using a combination of genetics, transgenic plant approaches, analytical chemistry and in vitro biochemistry. We will describe two evolutionary innovations that led to this novel phenotype: a ‘flipped’ acylsucrose acylation pathway that creates pyranose ring acylated acylsucroses, which in turn are substrates for a cell wall invertase-like enzyme. This hydrolase cleaves the glycosidic linkage of these pyranose acylated acylsucroses, but fails to use cultivated tomato-like furanose ring acylated acylsucrose substrates. These studies illustrate how multiple evolutionary innovations have led to the generation of a completely new class of specialized metabolites.


 

Co-Authors

Daniel Lybrand – Michigan State University; Yann-Ru Lou – Michigan State University; Pengxiang Fan – Michigan State University; Anthony Schilmiller – Michigan State University; Robert Last – Michigan State University

Bryan Leong

Graduate student
Michigan State University

Presentation(s):

Send Email for Bryan Leong


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CS-11-2 - Evolutionary innovations enhance metabolic diversity: biosynthesis of acylglucoses in Solanum pennellii trichomes



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