Cell/Development/Systems

Abstract

CS-20-3 - Discovery and characterization of amino acid exporters through amino acid sensors

Monday, July 16
3:58 PM - 4:18 PM

Amino acids are the major form of assimilated nitrogen found within the long-distance transport systems of plants, hence often regarded as the “nitrogen currency”. The delivery of amino acids to the target tissues through the long-distance transport systems requires a number of transport steps across the plasma membrane. Many of the cellular amino acid importers from plants have been identified, and in many cases, their physiological roles have been documented. On the other hand, the amino acid exporters involved in the long distance transport have remained elusive for a long time due to the lack of appropriate screening systems. We have developed an array of protein-based sensors that can be used to detect the local concentration of amino acids. Using these sensors, we were able to identify amino acid exporters from the model plant Arabidopsis. Combined with the genetics tools available in this species, we have assigned specific transporters to important physiological processes such as shoot-root amino acid transport and seed filling. Our goal is to use our knowledge to create a novel method to optimize nitrogen transfer in crop plants. 


 

Co-Authors

Julien Besnard – Texas A&M University; Chengsong Zhao – Virginia Tech; Unnati Sonawala – Virginia Tech; Eva Eva Collakova – Virginia Tech; Jean-Christophe Avice – Département de Biologie, Université de Caen ; Guillaume Pilot – Virginia Tech

Sakiko Okumoto

Associate Professor
Texas A&M University

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CS-20-3 - Discovery and characterization of amino acid exporters through amino acid sensors



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