Genetics/Genomics

Abstract

CS-17-3 - Corn RHS2, an endogenous tassel-specific siRNA-mediated glyphosate inducible male sterility system for hybrid seed production

Monday, July 16
3:58 PM - 4:18 PM

Hybrid crops produce higher yields than their inbred parents due to heterosis. For high purity of hybrid seeds, it is critical to eliminate self-pollination. Manual or mechanical removal of male parts (such as detasseling in maize) is labor-intensive, fuel and time-consuming, and can cause physical damage to female plants, resulting in significant seed yield reductions. Many male-sterility systems either require a maintainer for male-sterile line propagation or are often affected by environmental factors. Roundup® Hybridization System (RHS) utilizes glyphosate to induce male sterility, which effectively eliminates the need for maintainer lines and removal of male parts for commercial hybrid seed production. The first-generation RHS (RHS1) is based on low expression of a glyphosate-insensitive 5-enolpyruvylshikimate-3-phosphate synthase (CP4 EPSPS) in pollen. This talk presents the second-generation RHS (RHS2) technology built on RNA interference (RNAi) combined with CP4 EPSPS. RHS2 utilizes endogenous male tissue-specific small interfering RNAs (mts-siRNAs) to trigger cleavage of the CP4 EPSPS mRNA specifically in maize tassels, resulting in glyphosate-sensitive male cells due to lack of the CP4 EPSPS protein. Male sterility is then induced by glyphosate application at the stages critical for pollen development, and the male-sterile plants are used as the female parent to produce hybrid seed. The endogenous mts-siRNAs are conserved across maize germplasms, and the inducible male sterility was replicated in representative germplasms through introgression of a CP4 EPSPS transgene containing the mts-siRNA target sequence. This technology combines the relative simplicity and convenience of a systemic herbicide spray methodology with targeted protein expression to create an inducible male sterility system for industrial production of row crop hybrid seeds in an environmentally-independent manner.


 

Co-Authors

Heping Yang – Monsanto Company; Youlin Qi – Monsanto Company; Jintai Huang – Monsanto Company; Sergey Ivashuta – Monsanto Company; Yuanji Zhang – Monsanto Company; Oscar Sparks – Monsanto Company; Jiyan Ma – Monsanto Company; Brook Van Scoyoc – Monsanto Company; Amy Caruano-Yzermans – Monsanto Company; Jennifer King-Sitzes – Monsanto Company; Xin Li – Monsanto Company; Aihong Pan – Monsanto Company; Marty Stoecker – Monsanto Company; Elizabeth Wiggins – Monsanto Company; Rita Varagona – Monsanto Company

Michael Goley, MS

Herbicide Trait Project Lead
Monsanto Company

Michael Goley is a Herbicide Trait Project Lead at Monsanto Company. Over the past 16 years he has worked in both the Regulatory and Biotechnology organization, and has led teams that have developed several next generation products, including dicamba tolerance in corn, and more recently FOPs tolerance and improved Roundup Hybridization technology in corn. Mike received his BS and MS degrees in Biology from Bucknell University in Lewisburg, PA. He resides in Saint Louis, MO with his wife and 2 children.

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CS-17-3 - Corn RHS2, an endogenous tassel-specific siRNA-mediated glyphosate inducible male sterility system for hybrid seed production



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