Abiotic Stress/Whole Plant Bio
CS-9-3 - Contribution of SAUR genes to natural variations of thermo-sensitivity in growth architecture in Arabidopsis
Sunday, July 15
3:58 PM - 4:18 PM
Responses of growth architecture to a moderate temperature change differ greatly among accessions of Arabidopsis thaliana. Although molecular genetics on the reference accession Col-0 have revealed molecular identities of some regulatory components in temperature responses, it is still largely unknown how diversity in thermo-responses is generated and used for adaptation.
We used natural accessions of Arabidopsis thaliana
to reveal genetic loci conferring the genetic diversity in growth architecture thermosensitivity. Genome Wide Association Study (GWAS) has yielded a major quantitative trait locus (QTL) for variation in thermosensitivity of growth architecture. The major QTL contains a cluster of genes in a sub-family of auxin responsive SAUR (Small Auxin Up Response). CRISPR/CAS9 knockout of single and multiple genes in the cluster indicates indicate that this gene cluster is important for temperature responses in leaf architecture. Analysis of transgenic lines with different haplotypes suggests that variations in both gene expression and protein activities contribute to variations of thermo-sensitivity in natural Arabidopsis accessions.
Jian Hua – Cornell University