Dhamar Gabriela Rodriguez
Plants frequently have to weather both biotic and abiotic stressors. Water stress, for instance, affects many aspects of the physiology of plants, thus, they have evolved protective mechanisms to ensure their survival when threatened by adverse environmental conditions. A major phytohormone, abscisic acid (ABA), plays an essential part in acting toward varied range of stresses like water stress. The transcription factor ABA-Insensitive5 (ABI5) is one key regulator of ABA signaling and stress response in Arabidopsis. Potential ABI5-interacting protein, such as the ABI Five Binding Protein 1 (AFP1) attenuates ABA signals by targeting ABI5 for ubiquitin-mediated degradation in nuclear bodies. Recently, an intergenic lncRNA has been identified within the AFP and the Nuclear Transport Factor 2 (NTF2) locus, hence we named as lincAFP1, which it is expressed under abiotic stress conditions; however, its function is unknown. In this study, we show the identification of two splicing variants (lincAFP1.1, lincAFP1.2), and have functions during abiotic stress in Arabidopsis thaliana. Overexpression and deficiency of lincAFP1, increases and decreases, respectively, the expression of its adjacent genes (AFP1, NTF2). In addition, plants with a deficiency of this gene showed a hypersensitive phenotype with a delay in the germination under the ABA treatments. Meanwhile, the overexpression of the gene result in plants with an insensitive phenotype to ABA treatment. Our results suggest that, lincAFP1 acts as a positive regulator of the AFP1 expression and therefore stimulates the tolerance to ABA stress. We are, now, interested in determining the level on which this regulation is carried out.
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