Genetics/Genomics

Abstract

CS-22-3 - A Genome-wide Association Study Reveals a Novel Regulator of Ovule Number and Fertility in Arabidopsis thaliana

Tuesday, July 17
9:13 AM - 9:33 AM

Since ovules contain the female gametophytes which are fertilized during pollination to initiate seed development, the number of ovules that are produced during flower development is an important determinant of total plant yield. A few molecular components have been identified that affect ovule development. Among these are genes that are involved in carpel margin meristem formation, the auxin pathway, ovule identity establishment, and ovule primordia initiation. However, the major regulators are still poorly understood. We used natural variation in Arabidopsis accessions to identify new genes involved in the regulation of ovule number. 180 Arabidopsis accessions were screened and found to have broad phenotypic variation that ranged from 39 ovules to 84 ovules per pistil. A Genome-Wide Association Study (GWAS) revealed several genomic regions that are associated with ovule number. T-DNA insertion lines in candidate genes from the most significantly associated loci were screened for ovule number phenotypes. The OVULE NUMBER ASSOCIATED 1 (ONA1) gene was found to have pleiotropic effects on plant fertility that include regulation of ovule number as well as male and female gametophyte development. Overexpression of ONA1 increased ovule number in a background-dependent manner and more than doubled the total number of flowers produced in all backgrounds tested, indicating that manipulation of ONA1 levels can be used to increase overall plant yield.


 

Co-Authors

Jing Yuan – Purdue University

Sharon A. Kessler

Purdue University

Presentation(s):

Send Email for Sharon Kessler


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CS-22-3 - A Genome-wide Association Study Reveals a Novel Regulator of Ovule Number and Fertility in Arabidopsis thaliana



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