Cell/Development/Systems

Abstract

CS -15-5 - Analysis of the roles of CSLD proteins during polarized plant cell wall deposition in Arabidopsis

Monday, July 16
2:23 PM - 2:43 PM

Plant cell expansion is governed by the deposition of new cell wall components, and cellulose microfibrils, the major load bearing components in plant cell walls, are deposited along one or more entire faces of a cell during diffuse growth. However, during tip growth, newly synthesized cell wall polysaccharides are deposited in a restricted region. Previously we have shown that the cellulose synthase-like D family protein, CSLD3, localizes to the tips of growing root hairs and provides essential cell wall synthase activity during root hair tip growth.  We have further demonstrated that fluorescently-tagged CSLD2/3/5 proteins localize to newly-forming cell plates in dividing cells, providing essential cell wall synthase activities for the de novo formation of these new cell walls during plant cell cytokinesis. We have also shown that CSLD5 is an unstable protein that is degraded in a cell-cycle dependent manner. Using a novel forward-genetic mutation screening approach, we have identified a number of mutants which reduce CSLD5 protein turnover in interphase cells, and will present recent progress in characterizing the nature of these mutants. We will also present recent results clarifying the identity of the polysaccharides synthesized by CSLD proteins using a combination of genetic rescue experiments with CSLD-CESA chimeric proteins, and in vitro biochemical reconstitution experiments with CSLD, CSLA, and CESA proteins expressed and purified from S. cerevisiae microsomal membranes.


 

Co-Authors

JIyuan Yang – University of Michigan; Gwangbae Bak – University of Michigan; Jonathon Combs – University of Michigan; Breeanna Urbanowicz – University of Georgia; Maria Pena – University of Georgia

Erik Nielsen

Associate Professor
University of Michigan

Presentation(s):

Send Email for Erik Nielsen


Assets

CS -15-5 - Analysis of the roles of CSLD proteins during polarized plant cell wall deposition in Arabidopsis



Attendees who have favorited this

Please enter your access key

The asset you are trying to access is locked. Please enter your access key to unlock.

Send Email for Analysis of the roles of CSLD proteins during polarized plant cell wall deposition in Arabidopsis