Biochemistry

Abstract

CS-1-1 - Nutrient sensing via O-GlcNAcylation in plants

Sunday, July 15
1:03 PM - 1:23 PM

Nutrient sensing is important to both plants and animals. One important nutrient sensing pathway is through O-linked N-acetylglucosamine transferases (OGTs), which modify and modulate target proteins using donor substrate UDP-GlcNAc derived from nutrients through the hexosamine biosynthetic pathway. Genetic studies have shown essential functions of O-GlcNAc modification in plants. However, the proteins and sites subject to this post-translational modification were largely unknown. Using lectin weak affinity chromatography to enrich modified peptides, followed by mass spectrometric analysis, we reported the first large scale proteomic identification of O-GlcNAc-modified proteins and sites in the model plant Arabidopsis thaliana (1). Our study generates a snapshot of the O-GlcNAc modification landscape in plants, indicating functions in many cellular regulation pathways and providing a powerful resource for further dissecting these functions at the molecular level. I will report a further improved method to enrich O-GlcNAc modified proteins, and our progress on understanding substrates, function and regulation of O-GlcNAcylation in plants.


 


1. Xu SL, Chalkley RJ, Maynard JC, Wang W, Ni W, Jiang XY, Shin K, Cheng L, Savage D, Hühmer AFR, Burlingame AL, Wang ZY. Proteomic analysis reveals O-GlcNAc modification on proteins with key regulatory functions in Arabidopsis. 2017. PNAS, PMID: 28154133.


 

Co-Authors

Zhi-yong Wang, Professor – Carnegie Institution for Science

Shouling Xu

Principle Investigator
Carnegie Institution for Science

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Send Email for Shouling Xu


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CS-1-1 - Nutrient sensing via O-GlcNAcylation in plants



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