Biochemistry

Abstract

CS-1-1 - Nutrient sensing via O-GlcNAcylation in plants

Sunday, July 15
1:03 PM - 1:23 PM

Nutrient sensing is important to both plants and animals. One important nutrient sensing pathway is through O-linked N-acetylglucosamine transferases (OGTs), which modify and modulate target proteins using donor substrate UDP-GlcNAc derived from nutrients through the hexosamine biosynthetic pathway. Genetic studies have shown essential functions of O-GlcNAc modification in plants. However, the proteins and sites subject to this post-translational modification were largely unknown. Using lectin weak affinity chromatography to enrich modified peptides, followed by mass spectrometric analysis, we reported the first large scale proteomic identification of O-GlcNAc-modified proteins and sites in the model plant Arabidopsis thaliana (1). Our study generates a snapshot of the O-GlcNAc modification landscape in plants, indicating functions in many cellular regulation pathways and providing a powerful resource for further dissecting these functions at the molecular level. I will report a further improved method to enrich O-GlcNAc modified proteins, and our progress on understanding substrates, function and regulation of O-GlcNAcylation in plants.


 


1. Xu SL, Chalkley RJ, Maynard JC, Wang W, Ni W, Jiang XY, Shin K, Cheng L, Savage D, Hühmer AFR, Burlingame AL, Wang ZY. Proteomic analysis reveals O-GlcNAc modification on proteins with key regulatory functions in Arabidopsis. 2017. PNAS, PMID: 28154133.


 

Co-Authors

Zhi-yong Wang, Professor – Carnegie Institution for Science

Shouling Xu, PhD

Principle Investigator
Carnegie Institution for Science

Shouling Xu

Dr. Xu received her ph.D in Biology with Dr. Joseph Kieber from the University of North Carolina at Chapel Hill in 2008. After completion of her degree, she did her joint postdoc work with Dr. Zhiyong Wang at Carnegie and Dr. Alma Burlingame, at UCSF mass spectrometry facility, focusing on charactering protein complexes and protein modifications using proteomics tools. She then did a one-year postdoc at ThermoFisher Scientific, focusing on targeted quantification and instrumentation. In January 2017, she was appointed as director of proteomics at Carnegie institution and as a PI. She has set up Carnegie mass spectrometry facility and has been collaborating with labs at plant biology department at Carnegie and plant biology labs at Stanford on deploying proteomics approaches to study biological questions. In addition, she also studies nutrient sensing in plants, particularly on O-GlcNAcylation modification, which plays a fundamental role in plant growth and development.

Presentation(s):

Send Email for Shouling Xu


Assets

CS-1-1 - Nutrient sensing via O-GlcNAcylation in plants



Attendees who have favorited this

Please enter your access key

The asset you are trying to access is locked. Please enter your access key to unlock.

Send Email for CS-1-1 - Nutrient sensing via O-GlcNAcylation in plants