395240 - E. coli Attachment to Various Sized Particles and their Impact on Transport

Tuesday, June 5
8:30 AM - 10:00 AM
Location: Lakeshore B
Co-Authors: Rachel McDaniel, 1400 North Campus Drive – South Dakota State University; Bruce Bleakley, Alfred Diary Science Hall – South Dakota State University

E. coli is the leading cause of water quality impairments within South Dakota’s streams and rivers. Physical factors, such as attachment of bacteria to various particle sizes, shear stress, and the velocity of water, affect bacteria fate and transport and are significant components in understanding the dynamics of bacteria within the water column. The goal of this study was to evaluate the attachment of E. coli to particles of various sizes and use this information to predict transport within a stream during base flow and stormflow conditions. Water samples were collected during base flow and during storm events every 30 minutes over a 5-hour period using an autosampler. Samples were collected from Skunk Creek, eastern South Dakota and analyzed for E. coli attachment to various particle sizes, including coarse (diameter ≥ 0.016 mm ), fine (0.016 > diameter ≥ 0.004 mm ), and very fine or unattached (diameter < 0.004 mm ). Preliminary results for a storm event show the average E. coli concentrations were 5.97 × 102, 6.43 × 102, and 34.7 × 102 cfu 100ml-1 for coarse, fine, and unattached fractions, respectively. Total E. coli concentrations over the 5-hour storm events ranged from 2.7 × 103 to 8.47 × 103 cfu 100ml-1 with an average of 4.71× 103 cfu 100ml-1. This is approximately five times base flow concentration of 10 × 102 cfu 100ml-1. The proportion of bacteria associated with clay and unattached fraction was highest (75%) followed by attachment to fine particles (14%) and finally coarse particles (11%). The estimated travel distance range for the particle sizes during the storm event was 16.3 to 21 miles for the unattached, 0.9 to 1.3 miles for fine particles, and 0.06 to 0.09 miles for coarse particles. Further analysis of data from additional storm events is currently being conducted.

Louis Yaovi Amegbletor

South Dakota State University


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395240 - E. coli Attachment to Various Sized Particles and their Impact on Transport

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