Environmental

Oral

394770 - Effect of woodchips bioreactors on E. coli concentrations and culturable bacteria in tile drainage water

Tuesday, June 5
8:30 AM - 10:00 AM
Location: Lakeshore B
Co-Authors: Rachel McDaniel, South Dakota State – South Dakota State University; Bruce Bleakley, South Dakota State – South Dakota State University

Woodchip bioreactors (WBs) are a promising strategy to reduce the amount of nutrient transport in tile drainage systems. Although WBs can be an effective method in removing nitrate (N), not much is known regarding the impact of these systems on microbes from tile drainage water. Therefore, the main objective of this study is to quantify the potential effect of WBs on the changes in E. coli concentrations and culturable bacteria from four synthetic source waters in steady flow condition. Four laboratory scale denitrifying woodchips receiving four different synthetic waters were used in this study. Peristaltic pumps at the inlets were used to control flow rates in the reactors. The synthetic waters were continuously passed through the bioreactors for 60 days and samples were taken 10 times over the course of the experiment and collected from both the influent and effluent as well as the midpoint. The results of microbial tests of WBs demonstrated a consistent reduction of E. coli with removal efficiency >40%. However, the culturable bacteria increased, as expected. These results provide evidence for the ability of denitrifying bioreactors to reduce E. coli concentrations, representing their broader versatility for improving water quality from tile drainage systems. Additional work is being conducted to examine the changes in N removal, total organic carbon, biological oxygen demand, and dissolved oxygen and how these variables correlate to bacterial concentrations.

Sara Mardani

South Dakota State university

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