Water, Wastewater & Stormwater

Oral

389314 - Bio-hydrogen production from antimicrobial interventions wastewater obtained from a Midwestern cattle slaughterhouse using dark fermentation

Monday, June 4
2:00 PM - 3:30 PM
Location: Greenway EF
Co-Authors: Bruce Dvorak, Lincoln, NE – University of Nebraska-Lincoln; Jeyam Subbiah, Lincoln, NE – University of Nebraska-Lincoln

The beef industry is one of the largest industries in the agricultural sector. Over the past decade, there has been changes in the cattle processing with the introduction of the antimicrobial intervention technologies that use organic acids such as lactic acid (LA) and peracetic acid (PAA) for microbial disinfection. Antimicrobial interventions produce wastewater streams with low pH (2.1-4.5) and high organic content (COD: 2300 – 47000 mg/L). The goal of this study was to evaluate the hydrogen and VFAs production from antimicrobial wastewater using dark fermentation under different initial pH levels (4.5, 5.5, 6.5, 7.5 and 8.5) and temperature levels (35°C, 45°C, 50°C and 55°C). Two wastewater sources from antimicrobial interventions in a cattle slaughterhouse containing LA and PAA were evaluated in 1 L batch dark fermentation reactors. The reactors were inoculated using heat-shocked anaerobic digester sludge. No hydrogen production was observed using the PAA wastewater at all temperatures. Using the LA wastewater, hydrogen production was observed only at temperatures 35°C and 45°C and pH levels 6.5, 7.5 and 8.5. The highest hydrogen production (5.46 L H2/L) was achieved at pH of 7.5 and 45°C. The main VFAs produced from LA wastewater fermentation were acetate, propionate and butyrate. The highest acetate (97.5 mM) and propionate (125.5 mM) concentrations were achieved at pH of 7.5 and 35°C. The highest butyrate concentration (52.4 mM) was achieved at pH 8.5 and 45°C. The COD reduction ranged between 11 and 40 % and up to 99.8% LA transformation was achieved. Microbial community analysis using the 16s rRNA is to be done to evaluate shifts in relative abundance of different microbial species.

Rami M. M Ziara

PhD Candidate
University of Nebraska-Lincoln

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389314 - Bio-hydrogen production from antimicrobial interventions wastewater obtained from a Midwestern cattle slaughterhouse using dark fermentation



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