Lipidomics enables global screening of the effect of a stimulus like exercise on lipid metabolism. Changes in the lipidome of Arabian horses during endurance racing and its influence on performance have been studied, but information is lacking for Thoroughbred racehorses. Our objective was to assess the effect of supra-maximal exercise on the lipidome of racehorses.
Four Thoroughbred geldings of similar body condition and age were used. Horses were housed in the same barn and fed the same diet. Each horse underwent treadmill exercise to fatigue at 115% of its V̇O2max (10.6±0.6m/s; 10% slope). Venous plasma samples were obtained before and immediately, 15 and 30mins post-exercise, and evaluated using an untargeted lipidomics approach at West Coast Metabolomics Center. Data was analyzed using principal components analysis and 1-way RM ANOVA (significance was set at p < 0.05).
965 plasma lipids were detected. Of these, 184 were “known” as their spectra and retention index are linked to library entries. After exercise, plasma levels of eight lipids changed significantly, likely reflecting the occurrence of lipolysis. Briefly, an increase of unsaturated fatty acids (11,14-eicosadienoic, 9-11-octadecadienoic and α-linoleic acids) and a decrease of saturated fatty acids (Icosanoic, heptadecanoic, lauric and myristic acids) and phospholipids (Sphingomyelin) were observed.
This pilot study provides valuable information regarding changes in the lipidome of Thoroughbreds associated with supramaximal exercise and will serve as a reference to guide future studies of the racehorse metabolome.
Washington State University
Thursday, June 14
5:15 PM – 5:30 PM
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