Methylglyoxal (MG), D-lactate and several inflammatory markers are increased in human diabetes and metabolic syndrome. The objective of this prospective cross-sectional study was to determine if concentrations of MG, D-lactate, L-lactate and the inflammatory markers, tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and macrophage chemoattractant protein-1 (MCP-1), differ between horses with and without insulin dysregulation, as determined by a combined glucose-insulin tolerance test (CGIT).
Client-owned horses were recruited. Horses with abnormal physical examination or complete blood cell count, incomplete data, or with signs of pituitary pars intermedia dysfunction were excluded from further testing. The CGIT was performed after overnight grain fasting. Serum samples were stored at -80°C and the biomarkers were tested via previously validated ELISA or colorimetric assays. Age, body condition score (BCS), cresty neck score (CNS), baseline glucose, baseline insulin and triglycerides were also recorded. Characteristics of horses categorized as having insulin dysregulation and of those that did not were compared.
Of the 32 horses that met inclusion criteria, 12 horses (37.5%) were determined as having insulin dysregulation. Age, BCS, baseline glucose, triglycerides, MG, D-lactate, L-lactate, TNF-α, IL-6 and MCP-1 did not differ significantly between the two groups of horses. In univariate analysis, baseline insulin was significantly associated with insulin dysregulation (P < 0.05), but not in multivariate logistic regression. Of note, horses with CNS ≥ 3 had 11 times higher odds of having insulin dysregulation (OR 11.3, 95% C.I. 2.04-63.08, P < 0.05). In this study, inflammatory markers, MG and D-lactate were not good predictors of insulin dysregulation.
Large Animal Internal Medicine Resident, MSc candidate
Western College of Veterinary Medicine, University of Saskatchewan
2016/19 Large Animal Internal Medicine Residency, Western College of Veterinary Medicine (University of Saskatchewan)
2016/19 MSc in Large Animal Clinical Sciences, Western College of Veterinary Medicine (University of Saskatchewan)
2015/16 Equine Internship, Brazos Valley Equine Hospital, Navasota, Texas
2014/15 Equine Internship, Sycamore Lodge Equine Hospital, The Curragh, Ireland
2013 Equine Theriogenology Internship, Standardbred breeding farm, Italy
2012 DVM University of Turin, Italy
Thursday, June 14
2:00 PM – 2:15 PM
Friday, June 15
1:30 PM – 1:45 PM
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