Equine

Research Abstract

E01 - Equine Urinary N-Acetyl-β-D-Glucosaminidase Assay Validation and Correlation with Other Markers of Kidney Injury

Thursday, June 14
10:15 AM - 10:30 AM
Location: WSCC 620

Detection of equine kidney injury in initial stages, when intervention is likely to be most successful, is hindered by limited markers of early renal damage in horses. N-acetyl-β-D-glucosaminidase (NAG), a lysosomal enzyme in renal tubular cells that is released into urine during tubular insult, has shown promise for early identification of acute kidney injury in humans and other species. The aims of this study were to validate an assay for urinary NAG (uNAG) in horses and to correlate uNAG index with other markers of renal tubular dysfunction. An enzymatic assay was validated using coefficients of variation (CV), spike and recovery, and linearity of dilution. Urine and plasma were collected from 7 nonazotemic and 7 azotemic horses. Spearman rank correlation and Wilcoxon rank sum tests were used to compare uNAG index with plasma creatinine, urinary fractional excretion of sodium (FENa), and urinary gamma-glutamyltransferase (uGGT) index.


Intra- and inter-run CV, percent recovery after spiking, and linearity of dilution were satisfactory. There were significant correlations between uNAG index and plasma creatinine (p-value < 0.05) and between uNAG index and FENa (p-value < 0.01).  Median uNAG indices were significantly higher (p-value < 0.05) in azotemic horses, in horses with increased FENa, and in horses with increased uGGT index. Urinary NAG can be measured in horses and shows correlation with current biomarkers of renal dysfunction.  Additional work is needed to determine the timing of increase in uNAG index relative to onset of kidney injury in horses and to evaluate clinical utility in equine patients.

Rosemary Bayless, DVM

Equine Internal Medicine Resident
Colorado State University College of Veterinary Medicine and Biomedical Sciences

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E01 - Equine Urinary N-Acetyl-β-D-Glucosaminidase Assay Validation and Correlation with Other Markers of Kidney Injury

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