Small Animal Internal Medicine

Research Abstract

GI17 - Prevalence of Clostridium perfringens Encoding netF Gene in Dogs with Acute and Chronic Gastrointestinal Diseases

Thursday, June 14
4:15 PM - 4:30 PM
Location: WSCC 618/619

The virulence of Clostridium perfringens is attributed to various toxins. Recently, the newly described pore-forming toxin NetF, that is associated with Clostridium perfringens type A, has been reported in dogs with acute hemorrhagic diarrhea syndrome (AHDS), but limited information is available about the role of this toxin in other intestinal diseases of dogs. The aim of this study was to evaluate the prevalence of Clostridium perfringens encoding the netF gene in fecal samples from dogs with acute and chronic gastrointestinal diseases.


 


Fecal samples from 324 dogs were analyzed for the abundance of the netF gene by quantitative real time PCR. Dogs were divided into the following groups: healthy (HC, n=130), acute hemorrhagic diarrhea (AHDS, n=42), acute non- hemorrhagic diarrhea (AD, n=41), chronic enteropathy (CE, n=95), and dogs with exocrine pancreatic insufficiency (EPI, n=16). The proportions of dogs that had fecal samples positive for the netF gene were statistically analyzed using a Chi-square test (Graphpad Prism v7.0), and significance was set at p < 0.05.


 


A total of 20 fecal samples from dogs with gastrointestinal diseases were positive for Clostridium perfringens encoding the netF gene: 19/42 (45%) dogs with AHDS and 1/16 (6%) dogs with EPI. Fecal samples from all healthy dogs, dogs with CE, and dogs with acute non- hemorrhagic diarrhea were below the detection limit of the PCR assay. There was a significant association between the presence of Clostridium perfringens encoding netF gene and AHDS (p < 0.0001).


 


In this study, fecal samples of dogs with acute hemorrhagic diarrhea were commonly positive for Clostridium perfringens encoding the netF gene. Also, Clostridium perfringens encoding the netF gene was not detected in healthy dogs, dogs with acute non-hemorrhagic diarrhea, or dogs with chronic enteropathy.

Fatima Sarwar, D.V.M. , M.Phil.

Graduate Assistant Research
Gastrointestinal Laboratory, College of Veterinary Medicine, Texas A&M University, College Station, Texas

i completed the Doctor of veterinary medicine (DVM) and M. Phil Microbiology (2015) from the University of Agriculture, Faisalabad, Pakistan. Soon after that I got a fellowship and joined Gastrointestinal laboratory, College of veterinary medicine, Texas A & M University in fall 2016. Currently, i am a second year PhD student. My research interest includes alterations of gastrointestinal microbiota across healthy and diseased dogs and cats and their use as potential biomarkers for the diagnosis of gastrointestinal disease conditions.

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