Canine chronic enteropathies (CE) are associated with an excessive release of proinflammatory mediators (e.g., cytokines). In human and animal studies, cytokines such as interleukin (IL) -2, IL-6, IL-8, and tumor necrosis factor-alpha (TNF-α) have all been reported to be involved in the induction and maintenance of CE. The aim of this study was to describe serum concentrations of IL-2, IL-6, IL-8, and TNF-α in dogs with CE.
Twenty-five healthy dogs (CTRL) and 68 dogs with CE were included in this study. Serum IL-2, IL-6, IL-8, and TNF-α concentrations were measured using previously analytically validated electrochemiluminescence immunoassays (Canine Proinflammatory Panel 3 Ultrasensitive kit, Meso Scale Discovery). Data were analyzed using nonparametric statistics, receiver operating characteristic analysis (ROC), and principal component analysis (PCA). Significance was set at p < 0.05.
The CE group was composed of 46 males and 22 females, the median age was 6.3 years (min-max: 1-16 years). No significant differences for age or sex were identified between CE and CTRL dogs.
Serum IL-2, IL-6, and TNF-α concentrations were significantly increased in the CE group when compared to CTRL dogs. Results for both groups are shown in the table below. No correlation was found between age, sex, and any of the serum cytokine concentrations. Using a cutoff value of > 14 pg/mL for IL-6, sensitivity for discriminating CE and CTRL dogs was 84% and specificity was 72%. Using a cutoff value of > 2.2 pg/mL for TNF-α, sensitivity was 76% and the specificity was 80%. The areas under the curves for IL-6 and TNF were 0.81 and 0.84, respectively. The first principal component of PCA explained 85.5% of the variance where TNF-α was the major contributor.
In conclusion, dogs with CE have increased concentrations of IL-2, IL-6, and TNF-α, but we were unable to identify any significantly altered concentrations for IL-8. Further studies are needed to assess the utility of these cytokines as diagnostic or prognostic markers in dogs with chronic enteropathies.
Graduate Assistant Researcher
Gastrointestinal Laboratory, College of Veterinary Medicine, Texas A&M University
Agostino studied veterinary medicine at the University of Bologna, Italy, where he graduated in March 2012. Before graduation, he joined a study abroad and a student internship program at the University of Caceres, Spain, where he started his undergraduate research and thesis. After few months spent at the Veterinary Teaching Hospital at the University of Bologna, Agostino joined the GI Lab in May 2013 to pursue his PhD. Ago is interested in new markers of inflammation, lymphocytic activation, and epithelial barrier dysfunction in dogs affected by IBD. He’s very interested in the relationship and network between the immune system and the intestinal microbiota.
Thursday, June 14
11:00 AM – 11:15 AM
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