Gastro intestinal nematodes (GIN) have a significant impact on small ruminant production. Co-infection with multiple species of parasites are common and different parasites have different pathogenesis so investigation of the different populations of GIN is important to plan control strategies, understand interactions between parasites and the host as well as surveillance of regional diversity and determination of the specific populations involved in anthelmintic resistance. Several methods of identification are available, such as fecal culture and morphologic examination of the larval stage of the parasite or PCR, however these methods are time consuming, have a low throughput and/or require specific expertise.
We originally developed deep amplicon sequencing of the nematode rDNA ITS-2 (the Nemabiome) to quantify the species composition of cattle GIN and we have recently adapted and validated its use in small ruminants. Field applications of the Nemabiome have included epidemiological studies of the nematode species affecting sheep flocks in Western Canada (fig.1) the identification of the parasite populations resistant to anthelmintics as a complement to the Fecal Egg Count Reduction Test (fig.2). The information gathered using deep amplicon sequencing provides important qualitative and quantitative information about the epidemiology and resistance status of the different species of gastrointestinal parasites, which is essential to make informed decisions about treatment and control. We are currently developing the nemabiome approach to screen directly for anthelmintic drug resistance mutations in GIN populations.
Thursday, June 14
10:45 AM – 11:00 AM
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