Neurology

Research Abstract

N09 - Development of an Intraventricular Hemorrhage Model of Obstructive Hydrocephalus in Pigs

Thursday, June 14
12:15 PM - 12:30 PM
Location: WSCC 606/607

Ventriculoperitoneal shunt obstruction is a major complication in canine and human hydrocephalus, however obstruction typically occurs in the chronic setting. In order to develop a self-clearing ventricular catheter, a hydrocephalus model prone to early shunt obstruction would be beneficial. Autologous blood was injected into the right lateral ventricle of 32.3 ± 5.2 kg, male, 6 month old pigs during terminal (n=3) and survival (n=14) procedures. Median intraventricular intracranial pressures were 9 mmHg (baseline), 66 mmHg (peak) and 20 mmHg (6 minutes post-blood injection). 14.4 ml of blood caused profound hypertensive hydrocephalus in the terminal procedures but the first survival pig could not be recovered from anesthesia. Using 10 ml of blood, hypertensive hydrocephalus occurred in 12 of 13 pigs. In one pig, the subarachnoid space was inadvertently injected and hydrocephalus did not occur. Biweekly computed tomography for 6 weeks after survival procedures revealed blood injection alone failed to produce persistent hematoma (n=2) whereas injection of 10 ml of blood mixed with 140 units of thrombin produced persistent obstruction due to hematoma (n=10). Six pigs were additionally treated with ventriculoperitoneal shunting. Shunt obstruction by coagulated blood in 4 pigs (75%) caused profound ventricular dilation evident on computed tomography and post-mortem examinations. Acute neurological deterioration occurred 12 hours to 4 days post-operatively, and was fatal in 3 of the 4 pigs. A model of obstructive hydrocephalus prone to early shunt obstruction can be generated in 27 - 37 kg pigs with lateral ventricle injection of 10 ml of autologous blood and thrombin. 

R. Timothy Bentley, BVSc (Dist), MRCVS, DACVIM (Neurology)

Associate Professor, Veterinary Neurology
Veterinary Clinical Sciences, Purdue University

Liverpool University BVSc (Dist) 2005. Member of the Royal College of Veterinary Surgeons 2005. DACVIM (Neurology) 2009.
Rotating Small Animal Internship, Royal Veterinary College, University of London, 2005 - 2006.
Veterinary Neurology & Neurosurgery Residency, Tufts University, 2006 - 2009.
Purdue University, Veterinary Neurology & Neurosurgery: Assistant Professor (2009 - 2016), Associate Professor (2016 - Present).
At Purdue University, runs a multi-disciplinary brain tumor research program with focus upon canine glioma as a model for human glioma. Other research interests include developing application of MRI.

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