Electrochemotherapy (ECT) couples the administration of anticancer drugs with the delivery of electric pulses that increase the drug uptake through the cell membranes, thus resulting in an improved efficacy. This study has been undertaken to evaluate the tolerability and efficacy of the combination of systemic bleomycin and local cisplatin as ECT agents for incompletely excised canine soft tissue sarcoma (STS). Thirty dogs with incompletely excised STSs were enrolled. The dogs received intravenous 20 mg/m2 bleomycin and the tumor bed and margins were infiltrated with cisplatin at the dose of 0.5 mg/cm2, and then exposed to trains of permeabilizing pulses under sedation. More precisely, five minutes after the injection of the chemotherapy agents, sequences of 8 biphasic pulses lasting 50+50 microseconds each, were delivered in bursts of 1300 V/cm using caliper electrodes. A second session was performed two weeks later. The treatment was well tolerated and side effects were minimal. Twenty-six dogs had no evidence of recurrence at different times (remission range 6-36 months), four had recurrence and one of the four recurring dogs, died of lung metastases. ECT using combination of bleomycin and cisplatin appears to be effective in the treatment of incompletely resected STSs in dogs and could be a useful addition to the current options in consideration of its low cost, limited toxicity, and ease of administration.
Enrico P. Spugnini is currently Consulting Veterinary Oncologist at the Queen Elena Cancer Institute of Rome, Italy. He is founder and CEO of Biopulse Srl, a company devoted to the development of electroporation instruments and protocols.
He received his DVM degree from the University of Perugia, Italy in 1992. He did his residency in Oncology at the North Carolina State University in the years 1994-1996. Dr Spugnini completed his Ph D program on electrochemotherapy at the Bulgarian Academy of Sciences in 2005. Since 2006 he is a Diplomate of the American College of Veterinary Internal Medicine (Specialty of Oncology) and since 2006 he is also Diplomate of the European College. Dr. Spugnini specializes in alternative methods of delivery for chemotherapy agents in companion animals, with particular emphasis on electrochemotherapy.
He has lectured, written and published extensively (95 articles on journals with impact factor). Dr Spugnini has served as editor of a book on electroporation published by Nova Science, NY. He is also author of several chapters in books. He is holder of an European Patent on electrochemotherapy. He is in the editorial board of several peer-reviewed scientific journals.
Thursday, June 14
5:00 PM – 5:15 PM
Friday, June 15
11:20 AM – 12:10 PM
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