Activated platelets contribute in humans to sepsis complications and to multiple organ failure. The aim of the prospective study was to determine, if platelets are activated in clinical cases of equine systemic inflammatory response syndrome (SIRS).
Adult horses and ponies fulfilling at least two criteria of an adapted SIRS-score were included. A standard human protocol measuring activated platelets and platelet-leukocyte-aggregates (PLA) with fluorescence flow cytometry in platelet-leukocyte-rich-plasma (PLRP) was established in horses. Activation of platelets was determined by increased presentation of CD62P and CD154 on platelets. Activation and PLA were measured before and after in vitro activation of platelets with collagen. Ten healthy adult horses and ponies served as controls. Statistical analysis included proof of normal distribution followed by two-way ANOVA and post-hoc Bonferroni tests.
The 19 included horses and ponies with SIRS had significantly more activated platelets and PLA in native PLRP than controls: CD62P 11.73 ± 3.74 % in SIRS and 1.74 ± 0.36% in controls (P = 0.0004); CD154 2.10 ± 0.91 % and 0.40 ± 0.08% respectively (P = 0.119); PLA 6.23 ± 1.18 % and 2.46 ± 0.32% respectively (P = 0.031). Six horses survived. There was a trend for more activation and PLA in non-surviving horses. Furthermore a trend for reduced in vitro activation with collagen was detected in the non-survivors.
Equine Clinic, Internal Medicine; Department of Veterinary Clinical Science; Justus-Liebig-University
Diplomate ECEIM in 2011.
Friday, June 15
1:45 PM – 2:00 PM
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