Equine

Research Abstract

E25 - A Comparison of the Stability of Equine Blood D-Lactate in Sodium Fluoride and Serum Tubes

Friday, June 15
1:30 PM - 1:45 PM
Location: WSCC 615

Continuation of glycolysis in erythrocytes after blood collection may cause an artifactual in vitro increase of methylglyoxal, and consequently of its metabolite D-lactate. Sodium fluoride (NaF) inhibits enolase in the glycolysis pathway. Our hypotheses were that equine blood collected in NaF tubes would result in both valid and lower D-lactate values when compared to serum tubes, and that D-lactate concentrations would be stable up to 6 months at -80°C.


Blood was collected in both serum and NaF tubes from 23 horses and kept on ice until centrifugation for separation of serum or plasma. Samples were frozen at -80°C until analysis (after 1, 2 and 6 months) with a previously validated commercial assay (D-Lactate Colorimetric Assay Kit, BioVision, Inc., Milpitas, CA, USA).


D-lactate values measured from blood collected in serum tubes had a mean (± SD) concentration of 0.26mmol/L (0.09), 0.27mmol/L (0.09) and 0.17mmol/L (0.07) after 1, 2 and 6 months, respectively. From the NaF tubes, the mean D-lactate concentrations were 0.05mmol/L (0.01), 0.06mmol/L (0.01) and 0.03mmol/L (0.01) after 1, 2 and 6 months, respectively. D-lactate was significantly different (P < 0.0001) between serum and NaF samples, and when comparing 6 months to 1 and 2 months within the NaF tube type (P < 0.0001).


Use of NaF tubes is recommended for equine blood collection for determination of D-lactate concentration, as use of serum tubes results in inflated and inaccurate values. Furthermore, longer-term storage (>2 months at -80⁰C) appears to result in sample degradation and should be interpreted with caution.

Valentina M. Ragno, DVM

Large Animal Internal Medicine Resident, MSc candidate
Western College of Veterinary Medicine, University of Saskatchewan

2016/19 Large Animal Internal Medicine Residency, Western College of Veterinary Medicine (University of Saskatchewan)
2016/19 MSc in Large Animal Clinical Sciences, Western College of Veterinary Medicine (University of Saskatchewan)
2015/16 Equine Internship, Brazos Valley Equine Hospital, Navasota, Texas
2014/15 Equine Internship, Sycamore Lodge Equine Hospital, The Curragh, Ireland
2013 Equine Theriogenology Internship, Standardbred breeding farm, Italy
2012 DVM University of Turin, Italy

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