Poster Presentation

Poster Presentation

PR01 - 3-D File Systems - Fact or Fiction? Evaluation of the Efficacy of Novel 3-D Instrumentation on Bacterial Reduction: A Comparative Study

Thursday, April 26
10:00 AM - 1:00 PM
Room: Exhibit Hall

Novel 3-D file systems possess a uniquely shaped design and movement sequence to promote the disinfection of complex root canal anatomy. This study compared the efficacy of newer 3-D shaping file systems with conventional rotary instrumentation on the reduction of Enterococcus faecalis from the mesial canals and isthmus of extracted mandibular molars. Thirty-four human mandibular molars were accessed and crowns were sectioned to establish a working length of 15 mm using a #15, 0.02 K-file (Lexicon). Teeth were then sterilized, inoculated with a standardized amount of E. faecalis (OG1RF), and incubated for 12 days under anaerobic conditions. Teeth were randomly divided into three experimental groups: I. XP-3D Shaper (Brasseler) II. TruShape 3-D Conforming Files (Dentsply) III. Endosequence (Brasseler). Four teeth were used as controls: Two without bacteria, negative control and two without instrumentation/irrigation positive. Mesial canals were instrumented to size #30, 0.06 taper using 15 strokes which each respective file system and 3 ml of saline as an irrigant solution. Samples were collected pre- and post-instrumentation by placing paper points in the isthmus, mesio-buccal, and mesio-lingual canals for 30 seconds. Total DNA was extracted and quantitative real-time PCR was performed. The results were statistically analyzed with one-way ANOVA (p < 0.05). The TruShape was the only file that demonstrated a statistical significant reduction in E. faecalis biofilm. There was no difference between XP-3D and Sequence files. In conclusion, TruShape promoted significantly more E. faecalis biofilm reduction when compared to the other systems.

Sara Markovic

Endodontic Resident
University of Florida

Completed my undergraduate degree in Liberal Arts at Tufts University. Provided mental health services to young adults before completing a master's degree in healthcare ethics at Loyola University Chicago. Attended New York University College Dentistry Class of 2014. Completed a general practice residency at New York Methodist Hospital prior to pursuing advanced specialty training in endodontics at University of Florida. A current second year resident at University of Florida Gainesville - I enjoy spending time with my family and dogs.

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Shannon Wallet

Associate Professor
University of Florida

Associate Professor in the Department of Oral Biology at University of Florida College of Dentistry. Involved in interprofessional and translational research at the college and the medical science center.

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Marianella Natera-Fernandez

Clinical Assistant Professor, Director of Pre-doctoral Endodontics.
University of Florida

Dr. Marianella Natera-Fernandez is a Clinical Assistant Professor in the Department of Endodontics and is the Director of Predoctoral Endodontics. Dr. Natera-Fernandez previously served as an assistant professor at Rutgers University where she received her Master’s Degree in Endodontics and taught both undergrad and post-graduate endodontic students. She received her first dental degree at the Universidad de Los Andes in Merida, Venezuela in 1995 and then enjoyed 12 years in private practice as a general dentist, with a simultaneous part-time faculty teaching assignment at Universidad Santa Maria for 7 years. Dr. Natera-Fernandez completed a fellowship in Endodontics here at the University of Florida in 2009 and was excited to return to UF in 2017 helping to lead Endogators to Academic Excellence!

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Eleonora Bakiri

Clinical Professor
University of Florida

Clinical Assistant Professor in the Department of Endodontics at University of Florida, Gainesville. Involved with the predoctoral teaching, research and intramural Faculty Practice. Worked in private practice for 15 years in Athens, Greece.

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Aria Sharma

Dental student

Dental student at University of Florida College of Dentistry. Aria is interested in the specialty of endodontics and has worked in numerous interprofessional research projects.

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Jacqueline Abranches

Associate Professor
University of Florida

Associate Professor in the Department of Oral Biology at University of Florida College of Dentistry. Involved in interprofessional and translational research at the college and the medical science center.

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Jose Lemos

Associate Professor

Associate Professor in the Department of Oral Biology at University of Florida College of Dentistry. Involved in interprofessional and translational research at the college and the medical science center.

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Roberta Pileggi

Chair and Program Director of the Department of Endodontics at University of Florida, Associate Dean of Advanced and Graduate Education, AAE Board of Director for District II
University of Florida

Chair and Program Director, Associate Dean of the Department of Advanced and Graduate Education, District III Board Director, Coda consultant, Director of the School of Advanced Dental Sciences at University of Florida, College of Dentistry.

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PR01 - 3-D File Systems - Fact or Fiction? Evaluation of the Efficacy of Novel 3-D Instrumentation on Bacterial Reduction: A Comparative Study



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