Poster Presentation

Poster Presentation

PR08 - Quantitative Analysis of the Antimicrobial Efficacy of Two Rotary Files

Thursday, April 26
10:00 AM - 1:00 PM
Room: Exhibit Hall

It is well established that chemomechanical instrumentation is one of the most important phases of endodontic therapy. The aim of this study was to evaluate the efficacy of two different types of rotary files available in the market on their ability to assist the removal of Enterococcus faecalis biofilm. Human mandibular molars were accessed, sectioned to a working length of 12 mm, sterilized, inoculated with a standardized amount of E. faecalis (OG1RF), and incubated under anaerobic conditions for 12 days. Teeth were divided into 2 groups (n=10): Group I: instrumented with ProTaper Gold and Group II instrumented with EdgeTaper Platinum. Both experimental groups were instrumented following the manufacture’s specifications and irrigated with 5 ml of 8.25% NaOCl, 17% EDTA, and 2% Chlorexidine with sonic activation. Two teeth were used as internal controls (negative control without bacteria; positive control without instrumentation/irrigation). Mesio-buccal and mesio-lingual canals were instrumented using the corresponding rotary file to a MAF 30. Samples were obtained pre- and post-instrumentation from the isthmus and mesial canals using paper points. Total DNA was extracted and quantitative real-time PCR was used to analyze biofilm content. Data was analyzed using one-way ANOVA, and although the ProTaper Gold demonstrated a higher percentage of biofilm reduction, there was no statistical significant difference between the systems. In conclusion, both rotary file systems reduced E. faecalis biofilm formation.

Francisco Gari

Francisco Gari
University of Florida

Second year resident at the University of Florida, Department of Endodontics. After completing residency I will be entering private practice in my hometown of Jacksonville, FL.

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Marianella Natera-Fernandez

Clinical Assistant Professor, Director of Pre-doctoral Endodontics.
University of Florida

Dr. Marianella Natera-Fernandez is a Clinical Assistant Professor in the Department of Endodontics and is the Director of Predoctoral Endodontics. Dr. Natera-Fernandez previously served as an assistant professor at Rutgers University where she received her Master’s Degree in Endodontics and taught both undergrad and post-graduate endodontic students. She received her first dental degree at the Universidad de Los Andes in Merida, Venezuela in 1995 and then enjoyed 12 years in private practice as a general dentist, with a simultaneous part-time faculty teaching assignment at Universidad Santa Maria for 7 years. Dr. Natera-Fernandez completed a fellowship in Endodontics here at the University of Florida in 2009 and was excited to return to UF in 2017 helping to lead Endogators to Academic Excellence!

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Eleonora Bakiri

Clinical Professor
University of Florida

Clinical Assistant Professor in the Department of Endodontics at University of Florida, Gainesville. Involved with the predoctoral teaching, research and intramural Faculty Practice. Worked in private practice for 15 years in Athens, Greece.

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Jacqueline Abranches

Associate Professor
University of Florida

Associate Professor in the Department of Oral Biology at University of Florida College of Dentistry. Involved in interprofessional and translational research at the college and the medical science center.

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Jose Lemos

Associate Professor

Associate Professor in the Department of Oral Biology at University of Florida College of Dentistry. Involved in interprofessional and translational research at the college and the medical science center.

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Shannon Wallet

Associate Professor
University of Florida

Associate Professor in the Department of Oral Biology at University of Florida College of Dentistry. Involved in interprofessional and translational research at the college and the medical science center.

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Roberta Pileggi

Chair and Program Director of the Department of Endodontics at University of Florida, Associate Dean of Advanced and Graduate Education, AAE Board of Director for District II
University of Florida

Chair and Program Director, Associate Dean of the Department of Advanced and Graduate Education, District III Board Director, Coda consultant, Director of the School of Advanced Dental Sciences at University of Florida, College of Dentistry.

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