Poster Presentation

Poster Presentation

PR12 - Epigenetic Regulation of Apical Periodontitis by Histone Demethylase Jmjd1c and Jmjd3 in Mice

Thursday, April 26
10:00 AM - 1:00 PM
Room: Exhibit Hall

Apical periodontitis (AP) occurs from bacterial infection of root canals and manifests with inflammatory signaling at periapex. By-products of oral bacteria, e.g., lipopolysaccharide (LPS), trigger pro-inflammatory cytokines release and bone destruction. We demonstrated that many physiological responses to stressful stimuli such as radiation or inflammation involve highly regulated epigenetic modifications regulated by histone demethylase such as Jmjd3 and Jmjd1c. In this study, we investigated the role of Jmjd1c and Jmjd3 in generation of AP in mice after pulp exposure. Dental pulp of maxillary first molars of Jmjd1c knockout (Jmjd1c-KO), Jmjd3-Cre Rosa knockout (Jmjd3-KO) and wild-type (WT) mice were exposed to induce AP. Apical lesion development was assessed by uCT analysis, and histological analysis. Pulp exposure led to AP formation in mice in 7 – 21 days, and the size of AP was notably increased in Jmjd1c-KO mice compared with the WT mice. H&E staining revealed strong infiltration of inflammatory cells to periapex in Jmjd1c-KO mice, which also demonstrated enrichment of macrophages stained with F4/80 marker. LPS mediated activation of NF-kB was significantly increased in BMDM from Jmjd1c-KO compared with WT mice. Likewise, the secretion of the pro-inflammatory cytokines was enhanced in the Jmjd1c-KO mice. These findings indicate that epigenetic regulation plays a critical role in immune responses to oral bacterial infection in the root canals, and that changes in chromatin structure by the JmjC-containing histone demethylases compromises the inflammatory responses and exacerbated development of AP.

Abdullah Alshaikh

PhD Candidate
University of California, Los Angeles

I completed my dental school training at King Saud University in 2014, and a 1-year Advanced Clinical Training (ACT) program in Endodontics at UCLA in 2015. I am currently a Masters of Science candidate in Oral Biology in Dr. Mo Kang’s laboratory at UCLA, where I study the epigenetic regulation of inflammatory cytokine transcription and oral immune response, with an emphasis on epigenetic regulation of apical periodontitis. I have had the honor of having my work published in the Journal of Endodontics.

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Jaeyoung Lee

Postdoctoral Scholar

I have a long standing passion to understand how several signaling pathway can modulate physiological intracellular processes. Signaling transduction in cells and identifying the functional role of novel protein was my best interest in the field of biology. Through doctoral training, I have learned about anti-inflammation mechanism and within this subject, a molecular mechanism of inflammasome-mediated sterile inflammation was my main research project. I had found several roles of novel proteins that are related to regulation of inflammation. I have recently defined the distinct role of deubiquitinating enzyme USP50 in regulation of inflammasome activation via deubiquitination of adaptor protein ASC. Our lab focused on the molecular mechanisms of cellular senescence and the role of telomerase and telomere DNA in controlling the replicative lifespan of human epithelial cells. Moreover, we are studying the effects of cellular transplantation in dental pulp for de novo regeneration of pulp tissues. We have developments novel project to investigate the epigenetic mechanisms of pulpal inflammation using KDM3C knockout mice.

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Wei Chen

Adjunct Assistant Professor
University of California, Los Angeles

Dr. Wei Chen finished his B.S degree in Biology in 1994 at Anhui Normal University in China. After that he earned his Master's degree in Cellular biology in 1997 and the Ph.D degree in Molecular Biology in 2000 . He then became a Postdoc at UCLA School of Dentistry, Section of Endodontics in 2012. In 2012 he became a research assistant and an adjunct assistant professor in 2016. Dr. Chen has published more than 50 seminal papers in high impact journals, such as Journal of Biological Chemistry, Oncogene, Carcinogenesis, Cell Death and Differentiation.

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Noor Khouqeer

Resident

I completed my dental school training at King Saud University, Riyadh, Saudi Arabia in 2014. I am currently an Endodontic Resident at UCLA and a Masters of Science candidate in Oral Biology in Dr. Mo Kang’s laboratory at UCLA. Where I study the epigenetic regulation of inflammatory cytokine transcription and oral immune response, with an emphasis on epigenetic regulation of apical periodontitis.

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Mo K. Kang

Professor, Chair of the Division of Constitutive and Regenerative Sciences, Chair of the Section of Endodontics, Jack A. Weichman Chair in Endodontic
University of California, Los Angeles

Mo Kwan Kang, M.S., Ph.D., D.D.S. is a professor and the chair of the Section of Endodontics and the Division of Constitutive & Regenerative Sciences at the UCLA School of Dentistry. Dr. Kang also holds a dual appointment with the Section of Oral Biology and is the first person to hold the Dr. Jack A. Weichman Chair in Endodontics. The current research in Dr. Kang’s laboratory is focused on oral mucosal diseases, including oral cancer, chemical- and radiation-induced oral mucositis, and aging-associated oral epithelial dysfunction. These studies were developed in his laboratory from extensive investigations of the mechanisms underlying cellular senescence (aging) and immortalization of normal human oral keratinocytes (NHOK)

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