The potential for generating new tissue of periodontal ligament cells (PDLCs) plays a key role in regeneration of periodontium, including alveolar bone and cementum. Due to the special location of PDLCs in vivo, like a cushion to burden the mechanical forces of mastication, recent researches prefer to study PDLCs activities by using several types of mechanical model in vitro to mimic this specific environment. This study aim to evaluate the effect of different mechanical forces on PDLCs in mechanical cell cultured model in vitro. In this work, the PDLCs were cultured on mechanical loading machine with 30 rpm, 60 rpm, 90 rpm and 120 rpm mechanical strength for three days, respectively. Negative control was PDLCs without mechanical loading. Cell proliferation was assessed by MTT assay. After osteogenesis induction for seven days, runt-related transcription factor 2 and bone gamma-carboxyglutamic acid-containing protein expression was analyzed by reverse transcription quantitative polymerase chain reaction. Alkaline phosphatase acivity and alizarin red S staining were measured to evaluate the osteogenesis and mineralization activity. PDLCs under mechanical loading exhibited statistical significantly less osteogenesis potential than the group without mechanical strength (p < 0.05). The results suggest that mechanical strength has an influence on PDLCs osteogenesis activities that it can reduce PDLCs osteogenesis potential.
Clinical Assistant Professor
Seoul National University Gwanak Dental Hospital
I'm clinical assistant professor of seoul national university, seoul, Korea. My major is endodontics, and I'm interested in pulp regeneration. I'm happy to attend AAE 2018
I am a graduate student from Yonsei University School of Dentistry and major in endodontics. I'm interested in pulp-dentin complex regeneration. I am so glad that I can have a chance to join in AAE.
I'm graduate student of yonsei university, seoul, Korea. My major is applied science, and I'm interested in pulp regeneration. I'm happy to attend AAE 2018
I received a bachelor's degree in dentistry from Yonsei University School of Dentistry and received a master's degree and doctor's degree in endodontics from the same university. Now I'm an assistant professor at Yonsei University, seoul, Korea. I'm interested in pulp-dentin complex regeneration, mainly doing angiogenesis researches.
I received a bachelor's degree in dentistry from Yonsei University School of Dentistry and received a master's degree and doctor's degree in endodontic treatment from the same university. I have been trained in endodontics at Penn and now I am working as a professor at Yonsei university.