Calcium silicate cements have been widely applied in regenerative endodontic treatments. Many studies have reported their superior biocompatibility, sealing ability and odontogenic/osteogenic potential, however, there are relatively less reports on their angiogenic activity. The aim of this study was to evaluate the effect of various calcium silicate cements on vascular endothelial growth factor (VEGF) release in human dental pulp cells (hDPCs). In addition, human umbilical vein endothelial cells (HUVECs) under the conditioned medium from hDPCs were used to investigate the effect of various calcium silicate cements on angiogenesis. hDPCs were cultured on ProRoot MTA, Endocem Zr, Retro MTA, Biodentine, EndoSequence BC Sealer and Dycal. VEGF expression was assessed by enzyme-linked immunosorbent assay. Capillary tube formation assay was performed to evaluate the effect of material-induced angiogenesis on HUVECs. There was a statistically significant difference in VEGF levels depending on the test materials and times (P < 0.05). VEGF levels of EndoSequence BC Sealer groups at 116 hours were significantly higher than that of other groups. The ProRoot MTA, EndoSequence BC Sealer, Biodentine, Retro MTA, Endocem Zr groups showed a significant increase in capillary tube formation compared to the Dycal group. However, there was no statistically significant difference in tube formation between the calcium silicate cement groups. In conclusion, calcium silicate cements can be expected to enhance angiogenesis when in direct contact with hDPCs.
I'm graduate student of yonsei university, seoul, Korea. My major is applied science, and I'm interested in pulp regeneration. I'm happy to attend AAE 2018
Clinical Assistant Professor
Seoul National University Gwanak Dental Hospital
I'm clinical assistant professor of seoul national university, seoul, Korea. My major is endodontics, and I'm interested in pulp regeneration. I'm happy to attend AAE 2018
-Dr. Hyunkyung-Yoo graduated from Kyunghee University in 2015 and is currently a postgraduate student at the department of Conservative Denitstry, Yonsei University College of Dentistry since 2015.
Thursday, April 26
10:00 AM – 1:00 PM
I received a bachelor's degree in dentistry from Yonsei University School of Dentistry and received a master's degree and doctor's degree in endodontics from the same university. Now I'm an assistant professor at Yonsei University, seoul, Korea. I'm interested in pulp-dentin complex regeneration, mainly doing angiogenesis researches.
I received a bachelor's degree in dentistry from Yonsei University School of Dentistry and received a master's degree and doctor's degree in endodontic treatment from the same university. I have been trained in endodontics at Penn and now I am working as a professor at Yonsei university.