Poster Presentation

Poster Presentation

PR27 - Determination of Virulence Among Clinical Isolates of Tannerella Forsythia Based on Macrophage Responses

Thursday, April 26
10:00 AM - 1:00 PM
Room: Exhibit Hall

Objective: Tannerella forsythia is an anaerobic gram-negative bacterium commonly isolated from endodontic infections. An investigation of the inflammatory potential of this species is required to better understand the the pathogenic potential of this species in periapical diseases. The aim of this in vitro study was to investigate two different clinical isolates of T. forsythia with respect to their ability to induce an inflammatory cytokine response in human macrophages. Methods: Two genetically different T. forsythia (UB 4 and UB 20) strains obtained from periodontitis patients at the UB Dental Clinic were analyzed for their ability to activate cytokine expression in macrophages. Briefly, quantitative real-time RT2-PCR array was employed to profile the expression of a panel of inflammatory cytokine genes in THP-1 (human monocyte cell line) derived macrophages stimulated with various clinical isolates of T. forsythia. Protein level of cytokines whose transcriptions were differentially regulated was determined in the cell supernatants by ELISA. Results: The RT2-PCR array results showed that the cxcl10 gene was significantly upregulated in macrophages in response to UB 20 compared to UB 4.  PCR-array result for cxcl10 gene was validated with qRT-PCR using cxcl10 specific primers. The ELISA results confirmed higher concentration of secreted CXCL10 chemokine in the UB 20 stimulated cells. Conclusions: There is diversity in the virulence among the tested strains of T. forsythia. It can be predicted that endodontic infections due to UB 20 (or related phylotypes) might increase the risk for a sever root canal inflammation.

Hajar Albanyan

resident
State University of New York at Buffalo

Dr. Albanyan earned her BDS degree from King Saud University in Riyadh, Saudi Arabia in 2012. She is currently a third year resident of advanced education of Endodontics residency program at the State University of New York at Buffalo, which is combined with the Master's of oral science program. Her research interest is microbiology.

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Hammam Fageeh

Clinical Instructor
University of New York at Buffalo

Dr. Fageeh earned his BDS degree from King Khalid University in Abha, Saudi Arabia in 2011. He was also awarded a master's degree in oral science as well as a clinical certificate in periodontics from University at Buffalo in 2017. He is currently working as a clinical instructor in the periodontics department at the State University of New York at Buffalo. His research intrest is microbiology.

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Sreedevi Chinthamani

Research Scientist
University of New York at Buffalo

Sreedevi Chinthamani completed her Phd from Sri Venkateswara University, Tirupathi, India in 2008. Then she moved to University at Buffalo as post-doc and she has been working on underlying mechanisms of ora diseases such as Sjogren's syndrome and bacterial induced periodontitis. her major interest to study the interaction of bacterial carbohydrates with innate immune cells and her findings have been published in reputed journals such as AJP & Plos one etc.

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Ashu Sharma

Professor
University of New York at Buffalo

Dr. Ashu Sharma is a professor of oral biology at University at Buffalo Dental school whose research focuses on the virulence mechanisms of oral pathogens.

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PR27 - Determination of Virulence Among Clinical Isolates of Tannerella Forsythia Based on Macrophage Responses



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