Poster Presentation

Poster Presentation

PR38 - Discover the Mechanism of FOXO1 in Epithelial Responses to P. Gingivalis and Oral Polymicrobial Infection

Thursday, April 26
10:00 AM - 1:00 PM
Room: Exhibit Hall

Pulpal and periodontal disease, both initiated by biofilm infection, may also have etiologic influences on the progression of the other disease. Porphyromonas gingivalis, a Gram-negative obligate anaerobic rod, is now recognized as strongly associated with endodontic infection. Forkhead box-O-1 (FOXO1) has been shown as a key transcription factor in the epithelial cell P. gingivalis interaction. The pathogenic mechanisms of oral polymicrobial infections have yet to be studied in detail. We hypothesize that FOXO1 responses in epithelial cell will be dependent on the nature of the polymicrobial infection. This table clinic aims to demonstrate how to discover the mechanism of FOXO1 in epithelial responses to P. gingivalis and oral polymicrobial infection. The epithelial cell polymicrobial infection model using P. gingivalis, Streptococcus gordonii (S.gordonii), Fusobacterium nucleatum or Streptococcus mitis was built firstly. The transcription activity of FOXO1 was measured with the Dual-Glo luciferase assay system. The Immunofluorescence was used to determine the location of FOXO1 in cells by confocal laser scanning microscopy. Quantitative PCR was to demonstrate the transcription level of FOXO1 related anti-oxidant and anti-apoptosis genes in P. gingivalis and oral polymicrobial infection model in the presence or absence of small-interfering RNA (siRNA)-mediated knockdown of FOXO1. The findings position FOXO1 as an important transcription factor involved in the dynamic interaction between oral polymicrobial infection and host cells and a viable adjuvant in therapeutic protection against pulpal and periodontal disease.

Qian Wang

Resident
Case Western Reserve University

Dr. Qian Wang received D.M.D & Ph.D in China, and is currently an Endodontics Resident in Case Western Reserve University. Her research interests focus on oral microbiology and immunology.

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Anita Aminoshariae

Associate professor
Case Western Reserve University

Dr. Aminoshariae is currently working as an Associate professor at Case western Reserve University School of Dental Medicine. She is Director of Predoctoral Endodontics. She is also a Diplomate of American Board of Endodontics.

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Andre Mickel

Associate professor
Case Western Reserve University

Dr. Andre Mickel is Chair of the Department of Endodontics and Director of the Postgraduate Endodontic Program.He earned his D.D.S. and M.S.D. from Case Western Reserve University, as well as a G.P.R. from Western Reserve Care System in Youngstown, Ohio. He is a Board Certified Diplomate of the American Board of Endodontics.

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Richard J. Lamont

Professor/Chair
University of Louisville

Dr. Richard J. Lamont is the Delta Dental Endowed Professor and Chair in Department of Oral Immunology and Infectious Diseases in University of Louisville . His research interests mainly include Oral Immunology and Infectious Diseases.

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PR38 - Discover the Mechanism of FOXO1 in Epithelial Responses to P. Gingivalis and Oral Polymicrobial Infection



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